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Rabbit Anti-Securin  antibody (bs-1881R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
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产品编号 bs-1881R
英文名称 Securin
中文名称 垂体肿瘤转化基因抗体
别    名 AW555095; C87862; Cut2; EAP 1; EAP1; ESP1 ASSOCIATED PROTEIN 1; Esp1-associated protein; hPTTG; MGC126883; MGC138276; Pds1; Pituitary tumor transforming 1; Pituitary tumor transforming protein 1; Pituitary tumor-transforming 1, isoform CRA_a; Pituitary tumor-transforming 1, isoform CRA_b; Pituitary tumor-transforming gene 1; Pituitary tumor-transforming gene 1 protein; PTTG 1; PTTG; PTTG1; PTTG1 protein; PTTG1_HUMAN; Pttg3; Securin; TUMOR TRANSFORMING 1; Tumor transforming protein 1; TUMOR-TRANSFORMING 1; Tumor-transforming protein 1; TUTR 1; TUTR1.  
Specific References  (1)     |     bs-1881R has been referenced in 1 publications.
[IF=1.636] Shimoi G et al.Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes.(2019)J Reprod Dev.  WB ;  Mouse.  
研究领域 肿瘤  细胞生物  免疫学  细胞周期蛋白  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Pig,Horse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 22-28kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PTTG1: 101-202/202 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The encoded protein is a homolog of yeast securing proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013].

Function:
Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.

Subcellular Location:
Cytoplasm. Nucleus.

Post-translational modifications:
Phosphorylated at Ser-165 by CDK1 during mitosis.
Phosphorylated in vitro by ds-DNA kinase.
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.

Similarity:
Belongs to the securin family.

SWISS:
O95997

Gene ID:
9232

Database links:

Entrez Gene: 9232 Human

Entrez Gene: 30939 Mouse

Entrez Gene: 64193 Rat

Omim: 604147 Human

SwissProt: O95997 Human

SwissProt: Q6IAL9 Human

SwissProt: Q3Y5K4 Mouse

SwissProt: Q5SRU0 Mouse

SwissProt: Q9CQJ7 Mouse

SwissProt: P97613 Rat

Unigene: 350966 Human

Unigene: 6856 Mouse

Unigene: 137183 Rat

Unigene: 271 Rat



垂体肿瘤转化基因PTTG可在多种肿瘤组织及部分正常组织细胞内表达较高,对促进细胞增殖、转化及肿瘤发生有重要作用。
产品图片
Protein: human colon carcinoma lysates at 30ug;
Primary: Rabbit Anti-Securin(bs-1881R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size : 23kD
Observed band size : 23kD
Protein: testis(mouse) lysate at 30ug;
Primary: rabbit Anti- Securin (bs-1881R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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