| 产品编号 | bs-1868R |
| 英文名称 | PDCD1LG2 Rabbit pAb |
| 中文名称 | 程序性死亡配体2（CD273）抗体 |
| 别 名 | B7DC; Btdc; CD273; PD-L2; PDCD1L2; PDL2; bA574F11.2; PD1L2_HUMAN; PDCD1LG2; PD-1 ligand 2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC (B7-DC); |
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Specific References (1) | bs-1868R has been referenced in 1 publications.
[IF=1.808] Asuman Feda Bayrak. et al. Association of immune response with overall and disease-free survival in laryngeal squamous cell carcinomas. AM J OTOLARYNG. 2022 Jul;43:103477 IHC ; Human.
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| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Cow) |
| 产品应用 | Flow-Cyt=1ug/Test,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29 kDa |
| 检测分子量 | 42 |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDCD1LG2: 41-150/273 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis. [provided by Alliance of Genome Resources, Nov 2021] Function: Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Subunit: Interacts with PDCD1. Subcellular Location: Isoform 3: Secreted (Probable). Isoform 2: Endomembrane system; Single-pass type I membrane protein. Isoform 1: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. Similarity: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9BQ51 Gene ID: 80380 Database links: Entrez Gene: 80380 Human Omim: 605723 Human SwissProt: Q9BQ51 Human |
| 产品图片 |
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PDCD1LG2) polyclonal Antibody, Unconjugated (bs-1868R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:HepG2.
Primary Antibody (green line): Rabbit Anti-PDCD1LG2 antibody (bs-1868R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
