| 产品编号 | bs-2095R |
| 英文名称 | Integrin alpha 1 Rabbit pAb |
| 中文名称 | 整合素α1抗体 |
| 别 名 | CD 49a; CD49a; CD49 antigen-like family member A; CD49a; CD49a antigen; Integrin alpha-1; ITA1_HUMAN; Itga 1; ITGA1; Laminin and collagen receptor; Very late activation protein 1; VLA 1; VLA-1; VLA1. |
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Specific References (3) | bs-2095R has been referenced in 3 publications.
[IF=4.85] Yao Ding. et al. 1,25D/VDR inhibits pancreatic β cell ferroptosis by downregulating FOXO1 expression in diabetes mellitus. CELL SIGNAL. 2022 Dec;:110564 IF ; Rat.
[IF=2.81] Zhang, Rui, et al. "Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells." Stem Cells International 2015 (2015). WB ; Rat.
[IF=2.741] Cui, Xiyao. et al. TMT-based proteomic analysis reveals integrins involved in the synergistic infection of reticuloendotheliosis virus and avian leukosis virus subgroup J. BMC VET RES. Bmc Vet Res. 2022 Dec;18(1):1-8 WB ; Chicken.
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| 研究领域 | 细胞生物 免疫学 信号转导 干细胞 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Cow,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 128 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Integrin alpha 1: 1001-1179/1179 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Alpha 1 integrin along with alpha 2, alpha L and alpha M has a unique inserted domain. Integrin alpha 1 is a receptor for laminin and collagen. The alpha1 subunit is also known as CD49a. CD49a associates with CD29 (beta 1 integrin), to form an alpha1 beta1 heterodimer, identified as the rat homologue to VLA1, which is involved in cellular adhesion to laminin and collagen. Function: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Subunit: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1. Interacts with RAB21. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain. SWISS: P56199 Gene ID: 3672 Database links: Entrez Gene: 3672 Human Entrez Gene: 109700 Mouse Omim: 192968 Human SwissProt: P56199 Human SwissProt: Q3V3R4 Mouse Unigene: 644352 Human Unigene: 482186 Mouse Unigene: 91044 Rat |
| 产品图片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Lane 3: U87MG (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Integrin alpha 1 (bs
Blank control: U937(blue). Primary Antibody: Rabbit Anti- Integrin alpha 1 antibody(bs-2095R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Go
Blank control: RSC96 cells(blue). Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody(bs-2095R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody:
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
