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Rabbit Anti-Integrin alpha 1  antibody (bs-2095R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-2095R
英文名称 Integrin alpha 1
中文名称 整合素α1抗体
别    名 CD 49a; CD49a; CD49 antigen-like family member A; CD49a; CD49a antigen; Integrin alpha-1; ITA1_HUMAN; Itga 1; ITGA1; Laminin and collagen receptor; Very late activation protein 1; VLA 1; VLA-1; VLA1.  
Specific References  (3)     |     bs-2095R has been referenced in 3 publications.
[IF=4.85] Yao Ding. et al. 1,25D/VDR inhibits pancreatic β cell ferroptosis by downregulating FOXO1 expression in diabetes mellitus. CELL SIGNAL. 2022 Dec;:110564  IF ;  Rat.  
[IF=2.81] Zhang, Rui, et al. "Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells." Stem Cells International 2015 (2015).  WB ;  Rat.  
[IF=2.741] Cui, Xiyao. et al. TMT-based proteomic analysis reveals integrins involved in the synergistic infection of reticuloendotheliosis virus and avian leukosis virus subgroup J. BMC VET RES. Bmc Vet Res. 2022 Dec;18(1):1-8  WB ;  Chicken.  
研究领域 细胞生物  免疫学  信号转导  干细胞  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat (predicted: Mouse,Cow,Horse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 128kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Integrin alpha 1: 1001-1179/1179 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Alpha 1 integrin along with alpha 2, alpha L and alpha M has a unique inserted domain. Integrin alpha 1 is a receptor for laminin and collagen. The alpha1 subunit is also known as CD49a. CD49a associates with CD29 (beta 1 integrin), to form an alpha1 beta1 heterodimer, identified as the rat homologue to VLA1, which is involved in cellular adhesion to laminin and collagen.

Function:
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.

Subunit:
Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1. Interacts with RAB21.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.

SWISS:
P56199

Gene ID:
3672

Database links:

Entrez Gene: 3672 Human

Entrez Gene: 109700 Mouse

Entrez Gene: 25118 Rat

Omim: 192968 Human

SwissProt: P56199 Human

SwissProt: Q3V3R4 Mouse

SwissProt: P18614 Rat

Unigene: 644352 Human

Unigene: 482186 Mouse

Unigene: 91044 Rat



产品图片
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Lane 3: U87MG (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Integrin alpha 1 (bs-2095R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 150 kD
Observed band size: 160 kD
Blank control: U937(blue).
Primary Antibody: Rabbit Anti- Integrin alpha 1 antibody(bs-2095R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-2095R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: RSC96 cells(blue).
Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody(bs-2095R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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