| 产品编号 | bs-1855R |
| 英文名称 | MMP15 Rabbit pAb |
| 中文名称 | 基质金属蛋白酶-15抗体 |
| 别 名 | Matrix metalloproteinase-15; MMP 15; MMP-15; MMP15; Matrix metallopeptidase 15 membrane inserted; Matrix metalloproteinase 15; Membrane type 2 Matrix Metalloproteinase; Membrane type matrix metalloproteinase 2; MT MMP 2; MT2 MMP; MT2MMP; MTMMP 2; MTMMP2; SMCP 2; SMCP2; MMP15_HUMAN. |
| 研究领域 | 肿瘤 免疫学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61/76 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP15: 501-600/669 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. [provided by RefSeq, Jul 2008]. Function: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Subcellular Location: Membrane; Single-pass type I membrane protein; Extracellular side. Tissue Specificity: Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle. Post-translational modifications: The precursor is cleaved by a furin endopeptidase (By similarity). Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P51511 Gene ID: 4324 Database links: Entrez Gene: 4324 Human Entrez Gene: 17388 Mouse Omim: 602261 Human SwissProt: P51511 Human SwissProt: O54732 Mouse Unigene: 80343 Human Unigene: 217116 Mouse |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-MMP15 (bs-1855R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Sample: Heart(Mouse) lysate at 30ug;
Primary: Anti- MMP15 (bs-1855R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:61 kD
Observed band size:61 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
