| 产品编号 | bs-1913R |
| 英文名称 | MMP8 Rabbit pAb |
| 中文名称 | 基质金属蛋白酶-8/胶原酶2抗体 |
| 别 名 | Matrilysin/matrix metalloproteinases-8; CLG 1; CLG1; Collagenase II; Collagenase 2; Collagenase neutrophil; HNC; Matrix metalloprotease 8; MMP8; MMP 8; MMP-8; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNLCL; neutrophil collagenase preproprotein; MMP8_HUMAN; Neutrophil collagenase; Matrix metalloproteinase-8; PMNL-CL. |
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Specific References (4) | bs-1913R has been referenced in 4 publications.
[IF=5.246] Yin Zhuang. et al. Exosomes Secreted by Nucleus Pulposus Stem Cells Derived From Degenerative Intervertebral Disc Exacerbate Annulus Fibrosus Cell Degradation via Let-7b-5p. Front Mol Biosci. 2021; 8: 766115 WB ; Human.
[IF=3.27] Oralova, V., et al. "Osteogenic Potential of the Transcription Factor c-MYB." Calcified Tissue International: 1-12. WB ; Mouse.
[IF=3.04] Sîrbulescu, Ruxandra F., et al. "Mature B cells accelerate wound healing after acute and chronic diabetic skin lesions." Wound Repair and Regeneration (2017). IHC-F ; Mouse.
[IF=2.35] Zhang, Chao-ying, et al. "Hydrogen sulfide suppresses the expression of MMP-8, MMP-13, and TIMP-1 in left ventricles of rats with cardiac volume overload." Acta Pharmacologica Sinica (2013). Rat.
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| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP8: 241-340/467 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] Function: Can degrade fibrillar type I, II, and III collagens. Subcellular Location: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix (Probable). Note=Stored in intracellular granules. Tissue Specificity: Neutrophils. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P22894 Gene ID: 4317 Database links: Entrez Gene: 4317 Human Entrez Gene: 17394 Mouse Omim: 120355 Human SwissProt: P22894 Human SwissProt: O70138 Mouse Unigene: 161839 Human Unigene: 16415 Mouse Unigene: 44474 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Bone marrow Lysates
Lane 2: Mouse Spleen Lysates
Lane 3: Mouse Uterus Lysates
Lane 4: Mouse Testis Lysates
Lane 5: Mouse NIH/3T3 cell Lysates
Lane 6: Rat Bone marrow Lysates
Lane 7: Rat Spleen Lysates
Lane 8: Rat Uterus Lysates
Lane 9: Rat Testis Lysates
Lane 10: Human Jurkat cell Lysates
Lane 11: Human A549 cell Lysates
Lane 12: Human HL-60 cell Lysates
Lane 13: Human MCF-7 cell Lysates
Lane 14: Human HepG2 cell Lysates
Primary: Anti-MMP8 (bs-1913R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38kDa
Observed band size: 60kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
