| 产品编号 | bs-1852R |
| 英文名称 | DUSP4 Rabbit pAb |
| 中文名称 | 丝裂原活化蛋白激酶磷酸酶-2抗体 |
| 别 名 | HVH2; MKP-2; MKP2; TYP; 2700078F24Rik; E130306H24Rik; DUS4_HUMAN; DUSP4; Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2 (MAP kinase phosphatase 2 | MKP-2); 3.1.3.16; 3.1.3.48; VH2; DUS4_MOUSE; |
| 研究领域 | 肿瘤 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Dog) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MKP-2: 301-394/394 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. Subunit: Hollow spherical complex composed of 24 subunits with pseudooctahedral symmetry, has a tetramer as the basic unit. Subcellular Location: Nucleus. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain. SWISS: Q13115 Gene ID: 1846 Database links: Entrez Gene: 1846 Human Entrez Gene: 319520 Mouse Omim: 602747 Human SwissProt: Q13115 Human SwissProt: Q8BFV3 Mouse Unigene: 417962 Human Unigene: 170276 Mouse Unigene: 392187 Mouse MKPs是一类丝氨酸/苏氨酸和酪氨酸双重底物特异性的磷酸酶 ,对于丝裂素活化蛋白激酶活性的调节起着十分重要的作用，可使丝裂素活化蛋白激酶上的苏氨酸/酪氨酸去磷酸化失活。目前研究发现MKPs分别有MKP-1、MKP-2、MKP-3及MKP4-6。 MKPs受MAPK信号通路中多种成分的诱导 ,决定了它与MAPK之间作用的特异性。 通过去磷酸化作用调节MAPK信号途径的活性 ,确保了细胞内信号的精确传递 ,参与了多种主要的细胞功能的调节。 |
| 产品图片 |
Sample:
RAW264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-DUSP4 (bs-1852R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-DUSP4 antibody (bs-1852R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
