| 产品编号 | bs-1827R |
| 英文名称 | Rabbit Anti-IL-11 antibody |
| 中文名称 | 白介素11抗体 |
| 别 名 | Interleukin-11; Adipogenesis inhibitory factor; AGIF; IL 11; IL11; Interleukin 11; Oprelvekin; IL11_HUMAN. |
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Specific References (2) | bs-1827R has been referenced in 2 publications.
[IF=5.555] Pengsen Wu. et al. IL-11 Is Elevated and Drives the Profibrotic Phenotype Transition of Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy. Front Endocrinol. 2022; 13: 846106 IHC ; Human.
[IF=2.376] Lifei Li. et al. GnRH agonist treatment regulates IL-6 and IL-11 expression in endometrial stromal cells for patients with HRT regiment in frozen embryo transfer cycles. Reprod Biol. 2022 Jun;22:100608 WB ; Human.
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| 研究领域 | 心血管 细胞生物 免疫学 神经生物学 干细胞 血管内皮细胞 淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,GuineaPig) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 19kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-11: 22-120/199 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production.
Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy. Function: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Subcellular Location: Secreted. Similarity: Belongs to the IL-6 superfamily. SWISS: P20809 Gene ID: 3589 Database links: Entrez Gene: 3589 Human Omim: 147681 Human SwissProt: P20809 Human Unigene: 467304 Human 白介素11，是一种多向性细胞因子。很多细胞都能合成IL11，如：内皮细胞、纤维母细胞、软骨细胞、滑膜细胞、成骨细胞、上皮细胞、神经元、胸腺等。正常的血清中，IL11表达很少或处在检测水平之下。对造血系统的功能包括：干细胞、巨核细胞、骨髓细胞、红细胞和B淋巴细胞的增生和发育。对造血系统之外也具有多相性的作用，有些是刺激和促进增生，有些是抑制作用。人、大鼠、小鼠的IL-11的前体有199个氨基酸。成熟形态为178个氨基酸。 |
| 产品图片 |
Sample: Testis (Mouse) Lysate at 30 ug
Primary: Anti- IL-11 (bs-1827R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Rat Kidney tissue lysates
Primary: Anti-IL-11 (bs-1827R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (bs-1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (bs-1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
