| 产品编号 | bs-1825R |
| 英文名称 | Inhibin beta B |
| 中文名称 | 抑制素βB/Inhibin β B抗体 |
| 别 名 | MGC157939; Activin A; Activin AB alpha polypeptide; Activin AB; Activin AB beta polypeptide; Activin B; Activin beta A chain; Activin beta B chain; ActivinA; ActivinAB; ActivinB; EDF; Erythroid differentiation protein; follicle stimulating hormone releasing protein; FRP; FSH releasing factor; FSH releasing protein; INHBA; INHBB; Inhibin; Inhibin beta 1; Inhibin beta 2; Inhibin beta A; Inhibin beta A chain; Inhibin beta A subunit; Inhibin beta B; Inhibin beta B chain; Inhibin beta B subunit; Inhibin βB. |
 |
Specific References (1) | bs-1825R has been referenced in 1 publications.
[IF=3.998] A. Ikeda. et al. Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep-Uk. 2020 Nov;10(1):1-14 IHC ; Mouse.
|
| 研究领域 | 神经生物学 信号转导 生长因子和激素 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Sheep,GuineaPig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13/45kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Inhibin beta B: 251-350/407 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq]. Subcellular Location: Secreted. Similarity: Belongs to the TGF-beta family. SWISS: P09529 Gene ID: 3625 Database links: Entrez Gene: 3625 Human Entrez Gene: 16324 Mouse Omim: 147390 Human SwissProt: P09529 Human SwissProt: Q04999 Mouse Unigene: 1735 Human Unigene: 3092 Mouse Unigene: 35074 Rat 抑制素是转化生长因子TGFβ超级家族，主要由雌性动物卵巢颗粒细胞和雄性动物睾丸的Sertoli细胞分泌.研究表明,抑制素具有内分泌和旁分泌物的作用:既可通过复杂的反馈机理,调节体液FSH水平。 |
| 产品图片 |
Sample:
Brain (Mouse) Lysate at 40 ug Primary: Anti- Inhibin beta B (bs-1825R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD 
Sample:
Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Breast tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Mouse Liver tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Rat Breast tissue lysates Lane 7: Human A431 cell lysates Lane 8: Human HepG2 cell lysates Primary: Anti- Inhibin beta B (bs-1825R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/45 kDa Observed band size: 46 kDa 
Sample:
A431(Human) Cell Lysate at 40 ug Primary: Anti- Inhibin beta B (bs-1825R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD 
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (bs-1825R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
