| 产品编号 | bs-1808R |
| 英文名称 | Haptoglobin |
| 中文名称 | 结合珠蛋白/触珠蛋白抗体 |
| 别 名 | haptoglobin; Bp; Haptoglobin alpha chain; Haptoglobin alpha(1S) beta; Haptoglobin alpha(2FS) beta; Haptoglobin beta chain; Haptoglobin, alpha polypeptide; Haptoglobin, beta polypeptide; HP; Hp2 alpha; HP2 ALPHA2; HPA1S; HPT; MGC111141; HPT_HUMAN. |
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Specific References (3) | bs-1808R has been referenced in 3 publications.
[IF=15.923] Changsheng Xing. et al. Microbiota regulate innate immune signaling and protective immunity against cancer. Cell Host Microbe. 2021 Apr;: IHC ; Mouse.
[IF=2.31] Kälsch, Julia, et al. "Bathing in carbon dioxide-enriched water alters protein expression in keratinocytes of skin tissue in rats." International Journal of Biometeorology (2016): 1-8. IHC-P ; Rat.
[IF=2.088] Wang G et al. PPAR-γ Promotes Hematoma Clearance through Haptoglobin-Hemoglobin-CD163 in a Rat Model of Intracerebral Hemorrhage. Behavioural Neurology, 2018, 1–7. WB ; Rat.
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| 研究领域 | 肿瘤 免疫学 生长因子和激素 激酶和磷酸酶 结合蛋白 糖蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27/43kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Haptoglobin beta chain: 251-350/406 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. Subunit: Tetramer of two alpha and two beta chains; disufide-linked. The Hemoglobin/haptoglobin complex is composed of a haptoglobin dimer bound to two hemoglobin alpha-beta dimers. Interacts with CD163. Subcellular Location: Secreted. Tissue Specificity: Expressed by the liver and secreted in plasma. DISEASE: Anhaptoglobinemia (AHP) [MIM:614081]: A condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Congenital haptoglobin deficiency is a risk factor for anaphylactic non-hemolytic transfusion reactions. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains. SWISS: P00738 Gene ID: 3240 Database links: Entrez Gene: 3240 Human Entrez Gene: 15439 Mouse SwissProt: P00738 Human SwissProt: Q61646 Mouse |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 40 ug Primary: Anti-Haptoglobin (bs-1808R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27/43 kD Observed band size: 45 kD 
Sample:
Liver (Mouse) Lysate at 40 ug Primary: Anti-Haptoglobin (bs-1808R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27/43 kD Observed band size: 27 kD 
Sample:
Lane 1: Mouse Plasma Lane 2: Recombinant human Haptoglobin protein(bs-43006P)at 50ng Primary: Anti-Haptoglobin (bs-1808R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27/43kDa Observed band size: 27/43kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
