客服热线:400-901-9800          客服QQ:4009019800          技术答疑     技术支持     质量反馈     联系我们
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-GLUR2 antibody (bs-1798R)
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:support@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-1798R
英文名称 GLUR2
中文名称 谷氨酸受体2抗体
别    名 Ionotropic Glutamate receptor 2; Gria2; Glur-2; GluR-B; Glur2; GluR-2; GluR-K2; Glutamate receptor 2; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA receptor subunit ; AMPA-selective glutamate receptor 2; AMPA 2; AMPA 2; AMPA selective glutamate receptor 2; AMPA-selective glutamate receptor 2; AMPA2; GluA2; GLUR 2; GluR B; GluR K2; GluR-2; GluR-B; GluR-K2; GLUR2; GLURB; Glutamate receptor 2; Glutamate receptor ionotropic AMPA 2; Glutamate receptor ionotropic; Gria2; GRIA2_HUMAN; HBGR2.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 细胞生物  免疫学  神经生物学  信号转导  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 97kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GLUR2:151-250/883 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing(CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene.

Function:
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Subunit:
Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4. Interacts with ATAD1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts binding to ATAD1 and to GRIP1 and leads to AMPAR complex disassembly. Interacts with PICK1 (via PDZ domain). Interacts with PRKCABP and GRIP2. Interacts with GRIA1 and SYNDIG1. Interacts with LRFN1. Found in a complex with GRIA1, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.

SWISS:
P42262

Gene ID:
2891

Database links:

Entrez Gene: 2891 Human

Entrez Gene: 14800 Mouse

Entrez Gene: 29627 Rat

Omim: 138247 Human

SwissProt: P42262 Human

SwissProt: P23819 Mouse

SwissProt: P19491 Rat

Unigene: 32763 Human

Unigene: 220224 Mouse

Unigene: 91361 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample: HepG2 Cell Lysate at 30 ug
Primary: Anti-GluR2 (bs-1798R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 117 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (bs-1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (bs-1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated(bs-1798R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ugl in 100 uL1X PBS containing 0.5% BSA(green).
Blank control: RSC96(blue) Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
版权所有 2016-2020 www.bioss.com.cn 北京博奥森生物技术有限公司 京ICP备05066980号 京公网安备110107000727号
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100