| 产品编号 | bs-1754R |
| 英文名称 | Rabbit Anti-EAR1 antibody |
| 中文名称 | 嗜酸性粒细胞阳离子蛋白抗体 |
| 别 名 | ECP1_MOUSE; Cytotoxic ribonuclease; Eosinophil cationic protein 1; Eosinophil secondary granule ribonuclease 1; EAR-1; Ribonuclease 3-1; RNase 3-1; eosinophil cationic protein 1 precursor; Ear1; ECP 1. |
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Specific References (3) | bs-1754R has been referenced in 3 publications.
[IF=25.841] Xuefeng Fei. et al. Neddylation of Coro1a determines the fate of multivesicular bodies and biogenesis of extracellular vesicles. J Extracell Vesicles. 2021 Oct;10(12):e12153 WB ; Mouse.
[IF=5.52] Park, Shin Yong, et al. "Peptidoglycan Recognition Protein 1 Enhances Experimental Asthma by Promoting Th2 and Th17 and Limiting Regulatory T Cell and Plasmacytoid Dendritic Cell Responses." The Journal of Immunology (2013). Mouse.
[IF=2.629] Yu Wen-Yan. et al. Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation. Evid-Based Compl Alt. 2022;2022:4571981 IHC ; Rat.
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| 研究领域 | 细胞生物 免疫学 通道蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Eosinophil cationic protein 1: 65-155/155 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Eosinophil derived neurotoxin (EDN) is a protein belonging to the ribonuclease (RNase) A superfamily. It has recently been found to have antiviral activity against respiratory syncytial virus and human immunodeficiency virus in vitro. Function: Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities. Subunit: Interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). In vitro interacts with and insert into lipid bilayers composed of dioleoyl phosphatidylcholine and dioleoyl phosphatidylglycerol. In vitro, tends to form amyloid-like aggregates at pH 3, but not at pH 5, nor 7. Subcellular Location: Secreted. Note=Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus. Similarity: Belongs to the pancreatic ribonuclease family. SWISS: P97426 Gene ID: 13586 Database links: Entrez Gene: 13586 Mouse
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| 产品图片 |
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Bone tissue lysates
Primary: Anti-EAR1 (bs-1754R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAR1) Polyclonal Antibody, Unconjugated (bs-1754R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-EAR1 antibody (bs-1754R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
