| 产品编号 | bs-1727R |
| 英文名称 | Rabbit Anti-ABCG4 antibody |
| 中文名称 | ABC膜转运蛋白抗体 |
| 别 名 | ATP binding cassette, subfamily G member 4; ABCG 4; ATP binding cassette subfamily G member 4; WHITE 2; WHITE2; ABCG4_HUMAN. |
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Specific References (2) | bs-1727R has been referenced in 2 publications.
[IF=3.05] Matsumoto, Koichi, et al. "Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood–cerebrospinal fluid barrier in human brain." Histochemistry and Cell Biology (2015): 1-15. IHC-P ; Human.
[IF=1.22] Ueno, Masaki, et al. "Blood–brain barrier and blood–cerebrospinal fluid barrier in normal and pathological conditions." Brain Tumor Pathology (2016): 1-8. IHC-P ; Human.
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| 研究领域 | 免疫学 神经生物学 转运蛋白 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 71kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ABCG4: 551-646/646 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
he ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes. ABCG4 protein is highly expressed in both human and mouse brain, it is a 646aa molecule in human (chr 11q23) and mouse. It is an integral membrane protein may be involved in macrophage lipid homeostasis. The ABCG4 protein’s abundant expression in brain and close evolutionary relationship to the other members of the subfamily suggests a potential role in cholesterol transport. Function: May be involved in macrophage lipid homeostasis. Subcellular Location: Membrane; Multi-pass membrane protein (Probable). Similarity: Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. Contains 1 ABC transmembrane type-2 domain. Contains 1 ABC transporter domain. SWISS: Q9H172 Gene ID: 64137 Database links: Entrez Gene: 64137 Human Entrez Gene: 192663 Mouse Omim: 607784 Human SwissProt: Q9H172 Human Unigene: 126378 Human Unigene: 101876 Mouse Unigene: 29008 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ABCG4) Polyclonal Antibody, Unconjugated (bs-1727R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
