| 产品编号 | bs-2477R |
| 英文名称 | Rabbit Anti-Macrophage Inflammatory Protein 1 gamma antibody |
| 中文名称 | 巨噬细胞炎症蛋白-1γ抗体 |
| 别 名 | MIP-1 gamma; Macrophage inflammatory protein 1-gamma; CCF18; CCL10; CCL9; Chemokine CC motif ligand 9; mMIP1g; SCYA10; SCYA9; Small inducible cytokine A9;MIP 1 gamma; MIP1 gamma; CCL9_MOUSE. |
 |
Specific References (1) | bs-2477R has been referenced in 1 publications.
[IF=6.68] Cottle, Denny L., et al. "Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis." Human Molecular Genetics (2014): ddu459. Mouse.
|
| 研究领域 | 心血管 细胞生物 免疫学 趋化因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CCL9: 22-122/122 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils may play a role in neoplasia and inflammatory host responses and can bind heparin. Its highest expression is found in the small intestine and peripheral blood cells. It is induced by interferon gamma, mitogens and interleukin 1 and belongs to the intercrine beta (chemokine CC) family. Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors. Subcellular Location: Secreted. Tissue Specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain Similarity: Belongs to the intercrine beta (chemokine CC) family. SWISS: P51670 Gene ID: 20308 Database links: Entrez Gene: 20308 Mouse SwissProt: P51670 Mouse Unigene: 416125 Mouse
|
| 产品图片 |
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- MIP-1 gamma Polyclonal Antibody, Unconjugated(bs-2477R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti- Macrophage Inflammatory Protein 1 gamma Polyclonal Antibody (bs-2477R) at 1:50 for two hours at room temperature (green channel). 5
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
