| 产品编号 | bs-1699R |
| 英文名称 | Galectin 9 Rabbit pAb |
| 中文名称 | 半乳糖凝集素9抗体 |
| 别 名 | LGALS9; 36 kDa beta-galactoside-binding lectin; Ecalectin; Gal-9; Galectin-9; galectin9; HOM HD 21; HOMHD21; HUAT; Lectin galactoside binding soluble 9; LEG9_HUMAN; LGAL S9; LGALS 9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; UAT; Urate transporter/channel; Urate transporter/channel protein; LEG9_MOUSE. |
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Specific References (1) | bs-1699R has been referenced in 1 publications.
[IF=32.621] Daley, Donnele, et al. "Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance." Nature Medicine (2017). IF(IHC-F) ; Mouse.
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| 研究领域 | 细胞生物 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Pig,Cow) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35-39 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Galectin: 51-150/353 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 9 is an S-type lectin and an eosinophil chemoattractant (ECA) produced by antigen-activated T cells. Galectin 9 is involved in apoptosis, is thought to be a prognostic factor for breast cancer and is also overexpressed in Hodgkin's disease tissue. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Multiple alternatively spliced transcript variants have been found for this gene. Subunit: Contains 2 galectin domains. Homodimer. Subcellular Location: Cytoplasm. Secreted. Note=May also be secreted by a non-classical secretory pathway. Tissue Specificity: Accentuated expression in liver and thymus of embryo, detected in embryonic heart, brain, lung, liver, and kidney. Highly expressed in adult thymus, small intestine, and liver, and to a lesser extent in lung, kidney, spleen, cardiac, and skeletal muscle. Barely detectable in brain and reticulocyte. The isoform Long is expressed exclusively in the small intestine. Similarity: Contains 2 galectin domains. SWISS: O08573 Gene ID: 16859 Database links: Entrez Gene: 3965 Human Entrez Gene: 16859 Mouse Omim: 601879 Human SwissProt: O00182 Human SwissProt: O08573 Mouse Unigene: 81337 Human Unigene: 341434 Mouse Unigene: 10706 Rat |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Galectin 9 (bs-1699R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35-39 kD
Observed band size: 39 kD
Sample:
NIH/3T3 Cell Lysate at 30 ug
Primary: Anti-Galectin 9 (bs-1699R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35-39 kD
Observed band size: 35kD
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Galectin 9 antibody (bs-1699R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
