| 产品编号 | bs-1524R |
| 英文名称 | Rabbit Anti-GlyR alpha 1 + 2 + 3 antibody |
| 中文名称 | 甘氨酸受体alpha 1/2/3型抗体 |
| 别 名 | glycine receptor alpha 1; glycine receptor alpha 2; glycine receptor alpha 3; GLRA1; GLRA2; Glycine receptor 48 kDa subunit; Glycine receptor alpha 2; Glycine receptor subunit alpha 3; Glycine receptor subunit alpha 2; Glycine receptor, alpha 1 subunit; Glycine receptor, alpha 2 subunit; MGC138878; MGC138879; STHE; alpha 1 Glycine Receptor; GLRA1; GLRA1_HUMAN; Glycine receptor 48 kDa subunit; Glycine receptor alpha 1; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1; Glycine receptor, alpha 1 subunit; HKPX1; STHE; GLR; Glra2; GLRA2_HUMAN; Glycine receptor alpha 2 polypeptide; Glycine receptor alpha 2 subunit; Glycine receptor subunit alpha 2; Glycine receptor subunit alpha-2; Gycine receptor alpha 2. |
| 研究领域 | 免疫学 神经生物学 通道蛋白 细胞膜受体 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GlyR alpha 1: 101-200/449 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain. Subcellular Location: Cell Membrane SWISS: Q6DJV9 Gene ID: 2742 Database links: Entrez Gene: 2741 Human Entrez Gene: 14654 Mouse Omim: 138491 Human SwissProt: P23415 Human SwissProt: Q64018 Mouse Unigene: 121490 Human Unigene: 89320 Mouse Unigene: 10109 Rat 甘氨酸受体alpha 1是中枢神经系统中的重要抑制性离子通道受体，是哺乳类中枢神经系统中神经递质受体蛋白，属于配体门控离子通道超家族成员. |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Lung tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Cerebellum tissue lysates
Lane 7: Rat Testis tissue lysates
Lane 8: Human HeLa cell lysates
Lane 9: Human A431 cell lysates
Lane 10: Human SH-SY5Y cell lysates
Primary: Anti-GlyR alpha 1 + 2 + 3 (bs-1524R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 53 kDa
Sample:
Spinal cord (Mouse) Lysate at 40 ug
Spinal cord (Rat) Lysate at 40 ug
Primary: Anti-GlyR alpha 1 + 2 + 3 (bs-1524R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 54 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GlyR alpha 1/2/3 Polyclonal Antibody, Unconjugated(bs-1524R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
