产品编号	bsm-1623M
英文名称	CEA(C3) Mouse mAb
中文名称	癌胚抗原单克隆抗体（包被）
别名	Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100.
	Specific References (12) \| bsm-1623M has been referenced in 12 publications. [IF=8.008] Yuqi Xu. et al. Surface Defect-Involved and Single-Color Electrochemiluminescence of Gold Nanoclusters for Immunoassay. ANAL CHEM. 2022;94(35):12070–12077 IA ; Other. PubMed:35994734 [IF=8.008] Li Fu. et al. Coreactant-free and Near-Infrared Electrochemiluminescence Immunoassay with n-Type Au Nanocrystals as Luminophores. ANAL CHEM. 2022;94(34):11934–11939 IA ; Other. PubMed:35976331 [IF=7.48] Zong, Chen, et al. "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." Biosensors and Bioelectronics 43 (2013): 372-378. ELISA ; PubMed:23356995 [IF=6.41] Song, Jie, et al. "Using silver nanocluster/graphene nanocomposite to enhance photoelectrochemical activity of CdS: mn/TiO 2 for highly sensitive signal-on immunoassay." Biosensors and Bioelectronics (2016). other ; PubMed:26901458 [IF=5.83] Ren, Kewei, et al. "Target-Driven Triple-Binder Assembly of MNAzyme for Amplified Electrochemical Immunosensing of Protein Biomarker." Analytical Chemistry (2015). other ; PubMed:25557949 [IF=5.83] Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415. other ; Human. PubMed:22320247 [IF=5.7] Yu, Xiaoping, et al. "Motor-based Autonomous Microsensor for Motion and Counting Immunoassay of Cancer Biomarker." Analytical Chemistry (2014). other ; Human. PubMed:24731140 [IF=5.7] Yu, Qiaoling, et al. "Plasma-enhanced Antibody Immobilization for the Development of a Capillary-based Carcinoembryonic Antigen Immunosensor Using Laser-induced Fluorescence Spectroscopy." Analytical Chemistry ELISA ; Human. PubMed:23547735 [IF=5.123] Song X et al. Multiplex immunoassays using surface modification-mediated porous layer open tubular capillary.Anal Chim Acta. 2018 Dec 28;1043:1-10. LIF detection&ELISA ; Human. PubMed:30392656 [IF=4.52] Zhao, Changrong, et al. "Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition." Analytica Chimica Acta (2014). other ; PubMed:25261898 [IF=4.51] Wang, Jiamian, et al. "Fabrication of highly catalytic silver nanoclusters/graphene oxide nanocomposite as nanotag for sensitive electrochemical immunoassay." Analytica chimica acta 906 (2016): 80-88. ELISA ; PubMed:26772127 [IF=3.101] Cao Y et al. Detection of three tumor biomarkers in human lung cancer serum using single particle ICP-MS combined with magnetic immunoassay. Spectrochimica Acta Part B: Atomic Spectroscopy, 105797 (2020). Sandwich-type magnetic immunoassay ; Human. PubMed:doi:10.1016/j.sab.2020.105797
研究领域	肿瘤细胞类型标志物
抗体来源	Mouse
克隆类型	Monoclonal
克隆号	C3
交叉反应	Human
产品应用	IHC-P=1:100-500,IHC-F=1:100-50javascript:;0,IF=1:100-500,ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	150-200 kDa
检测分子量
细胞定位	细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human CEA/CD66e/CEACAM5: full length
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M PBS (pH7.4) with 0.02% Proclin300.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. Function: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Subunit: Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Post-translational modifications: Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. Similarity: Belongs to the immunoglobulin superfamily. CEA family. Contains 7 Ig-like (immunoglobulin-like) domains. SWISS: P06731 Gene ID: 1048 Database links: Entrez Gene: 1048 Human Omim: 114890 Human SwissProt: P06731 Human Unigene: 709196 Human CEA是一种胚胎性抗原，主要存在于胎儿消化道上皮组织，胰腺和肝癌中。CEA广泛存在各种上皮性肿瘤，尤其是胃肠道恶性肿瘤，CEA分布阳性类型与肿瘤的恶性度有关。CEA与EMA一样可作为上皮性恶性肿瘤的重要标记。分子量为150-200 kDa。该抗体与bsm-1624M（检测）（Mouse Anti-Human CEA Monoclonal）配对使用；主要用于ELISA试剂盒，胶体金试剂条制备生产都很好。大量订购可来电商议。
产品图片	Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA(C3)) Polyclonal Antibody, Unconjugated (bsm-1623M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序