| 产品编号 | bs-1514R |
| 英文名称 | Rabbit Anti-CD33 antibody |
| 中文名称 | CD33抗体 |
| 别 名 | CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor. |
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Specific References (5) | bs-1514R has been referenced in 5 publications.
[IF=5.97] Jiang et al. Involvement of p38 in signal switching from autophagy to apoptosis via the PERK/eIF2α/ATF4 axis in selenite-treated NB4 cells. (2014) Cell.Death.Dis. 5:e1270 IHC ; Mouse.
[IF=4.46] Shi, Kejian, et al. "Sodium selenite alters microtubule assembly and induces apoptosis in vitro and in vivo." Journal of hematology & oncology 6.1 (2013): 1-9. WB ; Human.
[IF=4.24] Shan, Wulin, et al. "LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo." The International Journal of Biochemistry & Cell Biology (2015). IHC-P ; Mouse.
[IF=3.057] Katarzyna Sikorskaet al. The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells. Materials (Basel)
. 2020 Jul 20;13(14):3227. FCM ; mouse.
[IF=2.936] Han Y et al. Chemical metabolomics for investigating the protective effectiveness of Acanthopanax senticosus Harms leaf against acute promyelocytic leukemia.RSC Adv., 2018, 8, 11983. IHC ; Mouse.
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| 研究领域 | 免疫学 干细胞 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40/37kDa |
| 检测分子量 | 67kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD33: 101-200/334 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome. [provided by Alliance of Genome Resources, Apr 2022] Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Subunit: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Monocytic/myeloid lineage cells. Post-translational modifications: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q63994 Gene ID: 12489 Database links: Entrez Gene: 945 Human Entrez Gene: 12489 Mouse Omim: 159590 Human SwissProt: P20138 Human SwissProt: Q63994 Mouse Unigene: 83731 Human Unigene: 140157 Mouse
CD33(Siglec-3)也是急性淋巴细胞白血病抗原,细胞表面跨膜糖蛋白.CD33表达于早期B淋巴细胞,某些粒性白细胞,骨髓基质细胞,部分上皮组织及其起源的肿瘤细胞中也有表达.主要应用于某些恶性淋巴瘤和白血病的分型. |
| 产品图片 |
Sample:
Lane 1: Bone (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD33 (bs-1514R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/37 kD
Observed band size: 65 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
