| 产品编号 | bs-1338R |
| 英文名称 | Fx1A Rabbit pAb |
| 中文名称 | 肾刷状缘抗体 |
| 别 名 | gp330; RTEα5; LRP2_RAT; Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 330; Megalin.; Fx1A; |
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Specific References (2) | bs-1338R has been referenced in 2 publications.
[IF=4.25] Feng et al. Na+/H+ exchanger-1 reduces podocyte injury caused by endoplasmic reticulum stress via autophagy activation. (2014) Lab.Invest. 94:439-54 IF ; Rat.
[IF=3.3] Wu, Lingling, et al. "Rapamycin upregulates autophagy by inhibiting the mTOR-ULK1 pathway, resulting in reduced podocyte injury." PloS one 8.5 (2013): e63799. other ;
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| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 518 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Fx1A protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Binding of anti-Fx1A to Heymann nephritis antigens (HA) on rat glomerular epithelial cells (GECs) in culture leads to capping and disappearance of antigens from the cell surface. This process may contribute to the formation of glomerular subepithelial immune deposits in vivo. The authors differentially extracted GECs to determine whether HA redistribution is mediated by cytoskeletal components. Observations were made by phase-contrast and immunofluorescence microscopy on primary and passaged GECs in monolayer culture and by spectrofluorimetry on GECs in suspension. Function: Acts together with cubilin to mediate HDL endocytosis. Receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B. Subunit: Binds plasminogen, extracellular matrix components, plasminogen activator-plasminogen activator inhibitor type I complex, apolipoprotein E-enriched beta-VLDL, lipoprotein lipase, lactoferrin, CLU/clusterin and calcium. Forms a multimeric complex together with a receptor-associated protein (RAP). Binds to ankyrin-repeat family A protein 2 (ANKRA2). Interacts with LRP2BP. Interacts (via NPXY motif) with DAB2; the interaction is not affected by tyrosine phosphorylation of the NPXY motif. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit. Cytoplasm. Note=Expressed in clathrin-coated pits, a soluble form is possibly derived by cleavage at the cell surface. Tissue Specificity: Epithelial cells of kidney glomerulus and proximal tubule, lung, epididymis, yolk sac, among others. Similarity: Belongs to the LDLR family. Contains 17 EGF-like domains. Contains 36 LDL-receptor class A domains. Contains 37 LDL-receptor class B repeats. Database links: Entrez Gene: 201456 Human Omim: 609093 Human SwissProt: Q8NCQ5 Human Unigene: 664011 Human gp330是肾小管上皮细胞刷状缘上的一种大分子脂蛋白，Fx1A；经生物化学方法纯化后称RTEα5，Fx1A为大分子糖蛋白，近来证实它是—种330Ka的糖蛋白，现称gp330。 主要与肾小球上皮细胞整合素受体作用的抗刷状缘抗体(抗FX1A) 。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fx1A) Polyclonal Antibody, Unconjugated (bs-1338R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fx1A) Polyclonal Antibody, Unconjugated (bs-1338R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20 minutes at 37°C, followed by a conjugated streptavidin (bs-0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
