| 产品编号 | bs-1614R |
| 英文名称 | Patched Rabbit pAb |
| 中文名称 | Patched/PTCH抗体 |
| 别 名 | Protein patched homolog 1; PTCH; PTC1; A230106A15Rik; BCNS; FLJ26746; FLJ42602; Holoprosencephaly 7; HPE7; mes; NBCCS; OTTHUMP00000021709; OTTHUMP00000021710; Patched; Patched(Drosophila) homolog; Patched 1; Patched homolog(Drosophila); Patched homolog 1(Drosophila); Patched homolog 1; Patched protein homolog 1; PTC; PTC1; PTCH; PTCH protein+12b; PTCH protein+4'; PTCH protein-10; PTCH protein; PTCH1; PTCH1 protein; PTCH11; Ptch2; ; Patched/PTCH; PTC1_HUMAN. |
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Specific References (2) | bs-1614R has been referenced in 2 publications.
[IF=6.244] Cai Y. et al. Study on the Mechanism of Sancao Tiaowei Decoction in the Treatment of MNNG-Induced Precancerous Lesions of Gastric Carcinoma Through Hedgehog Signaling Pathway.. FRONT ONCOL. 2022 May;12:841553-841553 IHC ; Rat.
[IF=2.47] Ma, Tao-tao, et al. "Geniposide alleviates inflammation by suppressing MeCP2 in mice with carbon tetrachloride-induced acute liver injury and LPS-treated THP-1 cells." International Immunopharmacology (2015). WB ; Human.
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| 研究领域 | 肿瘤 细胞生物 发育生物学 神经生物学 信号转导 干细胞 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 161 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Patched/PTCH: 581-680/1447 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome. Function: Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Subunit: Interacts with SNX17. Interacts with IHH. Subcellular Location: Membrane. Tissue Specificity: In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin. Post-translational modifications: Glycosylation is necessary for SHH binding. DISEASE: Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients. Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462]. Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Similarity: Belongs to the patched family. Contains 1 SSD (sterol-sensing) domain. SWISS: Q86XG7 Gene ID: 5727 Database links: Entrez Gene: 5727 Human Entrez Gene: 19206 Mouse Omim: 601309 Human SwissProt: Q13635 Human SwissProt: Q86XG7 Human SwissProt: Q61115 Mouse Unigene: 494538 Human Unigene: 228798 Mouse Unigene: 102312 Rat Ptch蛋白是细胞表面接受Hh信号蛋白的受体，目前主要用于肿瘤方面的研究。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
