| 产品编号 | bs-0396R |
| 英文名称 | Rabbit Anti-VCAM1 antibody |
| 中文名称 | 血管内皮细胞粘附分子（CD106）抗体 |
| 别 名 | VCAM1; CD 106; CD106; CD106 Antigen; DKFZp779G2333; INCAM 100; L1CAM; MGC99561; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; INCAM-100; V-CAM 1; VCAM1_HUMAN. |
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Specific References (16) | bs-0396R has been referenced in 16 publications.
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| 研究领域 | 肿瘤 心血管 细胞生物 细胞粘附分子 内皮细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1µg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 81kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant Mouse VCAM 1 protein <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells. Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. Subunit: Binds to ECMV-D capsid proteins and acts as a receptor for this virus. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. Similarity: Contains 7 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P29533 Gene ID: 22329 Database links: Entrez Gene: 7412 Human Entrez Gene: 22329 Mouse GenBank: NP_001069.1 Human GenBank: NP_001186763.1 Human Omim: 192225 Human SwissProt: P19320 Human SwissProt: P29533 Mouse
VCAM 1（CD106）是一种膜相关蛋白，分布于血管内皮及其周围的淋巴细胞、单核细胞、嗜酸性粒细胞和中性粒细胞。Anti-VCAM-1主要用于各种组织中血管内皮的检测。 |
| 产品图片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Primary: Anti-VCAM1 (bs-0396R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 100 kDa
Sample:
Lane 1: Siha (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-VCAM-1 (bs-0396R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kD
Observed band size: 110 kD
Sample: VCAM1 protein (Human) at 100 ng
Primary: Anti-VCAM-1 (bs-0396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated(bs-0396R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Mouse Spleen(blue). Primary Antibody:Rabbit Anti-VCAM-1 antibody (bs-0396R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0396R, 1μg /8x10^5 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
