| 产品编号 | bs-1618R |
| 英文名称 | Rabbit Anti-CD146 antibody |
| 中文名称 | 黑色素瘤细胞粘附分子CD146抗体 |
| 别 名 | A32 antigen; CD 146; CD146 antigen; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32; Melanoma associated antigen MUC18; Melanoma associated glycoprotein MUC18; Melanoma cell adhesion molecule; MelCAM; MUC 18; MUC18; S endo 1; S endo 1 endothelial associated antigen; Sendo 1 endothelial associated antigen; Sendo1; MUC18_HUMAN. |
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Specific References (4) | bs-1618R has been referenced in 4 publications.
[IF=9.933] Tino Stauber. et al. Extrinsic Macrophages Protect While Tendon Progenitors Degrade: Insights from a Tissue Engineered Model of Tendon Compartmental Crosstalk. 2021 Sep 08 IF ; mouse.
[IF=1.89] Hu, Jianguo, Biao Zeng, and Xingwei Jiang. "The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious."International journal of clinical and experimental pathology 8.2 (2015): 1525. IHC-P ; Mouse.
[IF=1.257] Hai Zhao. et al. Oxidative stress caused by a dysregulated Wnt/β-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome. Zygote. 2020 Oct;:1-8 WB,IHC ; Mouse.
[IF=0] Kanthilal, Manisha. Characterization of Molecular and Mechanical Phenotypes of Freshly Isolated Lipoaspirate Cells. Diss. Brown University, 2013. FCM ; Human.
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| 研究领域 | 肿瘤 免疫学 细胞粘附分子 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 71kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD146: 201-300/646 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion. Function: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 Ig-like V-type (immunoglobulin-like) domains. SWISS: P43121 Gene ID: 4162 Database links: Entrez Gene: 4162 Human Entrez Gene: 84004 Mouse Omim: 155735 Human SwissProt: P43121 Human SwissProt: Q8R2Y2 Mouse Unigene: 599039 Human Unigene: 275003 Mouse Unigene: 2694 Rat CD146分子主要是选择性的在肿瘤血管内皮细胞中表达较高。 |
| 产品图片 |
Sample:
Lane 1: Human Huvec cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human U2OS cell lysates
Lane 4: Human U251 cell lysates
Primary: Anti-CD146 (bs-1618R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 115 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
