| 产品编号 | bs-1475R |
| 英文名称 | Rabbit Anti-Gremlin antibody |
| 中文名称 | 骨形态形成蛋白拮抗蛋白抗体 |
| 别 名 | CKTSF1B1; GREMLIN-1; GREMLIN 1; GREMLIN1; Cysteine knot superfamily BMP antagonist 1; DAND2; DRM; GREMLIN; Gremlin1; Gremlin 1; Gremlin-1; IHG2; PIG2; proliferation inducing gene 2 protein. |
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Specific References (4) | bs-1475R has been referenced in 4 publications.
[IF=3.7] Wellbrock, Jasmin, et al. "Overexpression of Gremlin-1 in Patients with Loeys-Dietz Syndrome: Implications on Pathophysiology and Early Disease Detection." PLoS one 9.8 (2014): e104742. IHC-P ; Human.
[IF=2.094] He H et al. Low oxygen concentrations improve yak oocyte maturation and enhance the developmental competence of preimplantation embryos. Theriogenology. 2020 Oct 15;156:46-58. WB ; Yak.
[IF=1.817] Yoichiro Aoshima. et al. Gremlin-1 for the Differential Diagnosis of Idiopathic Pulmonary Fibrosis Versus Other Interstitial Lung Diseases: A Clinical and Pathophysiological Analysis. 2021 Mar 26 IHC ; Human.
[IF=0] KAWAGUCHI, Kenichiro, et al. "Role of Gremlins in the Aortic Arch of Spontaneously Hypertensive and Hyperlipidemic Rats." The Showa University Journal of Medical Sciences 28.2 (2016): 89-99. Rat.
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| 研究领域 | 肿瘤 免疫学 发育生物学 信号转导 干细胞 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human gremlin: 101-184/184 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In Xenopus, Gremlin 1 belongs to a novel gene family that act as BMP antagonists in embryonic explants. The human homolog of Gremlin 1(CKTFS1B1) is localized on human chromosome 15q13--> q15. Gremlin 1-specific mRNA is expressed in different human tissues, including brain, ovary, intestine and colon. It has been suggested that down-regulation of Gremlin 1 is associated with tumor progression, and support the hypothesis that human Gremlin 1 may play an important role during both neuroembryological development and carcinogenesis. Function: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Subunit: Interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Similarity: Belongs to the DAN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. SWISS: O60565 Gene ID: 26585 Database links: Entrez Gene: 26585 Human Entrez Gene: 23892 Mouse Omim: 603054 Human SwissProt: O60565 Human SwissProt: O70326 Mouse Unigene: 40098 Human Unigene: 166318 Mouse Unigene: 42929 Rat gremlin属于骨形态形成蛋白BMP拮抗剂家族成员,在胚胎发育过程中可通过调节BMP活性，影响器官、体征的形成和组织分化。 |
| 产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Gremlin (bs-1475R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
Sample:
intestine (Mouse) Lysate at 40 ug
skin (Mouse) Lysate at 40 ug
Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (bs-1475R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (bs-1475R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat rectum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (bs-1475R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated(bs-1475R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
