| 产品编号 | bs-1482R |
| 英文名称 | CD177 |
| 中文名称 | 嗜中性粒细胞抗原CD177抗体 |
| 别 名 | NB1 GP; PRV-1; HNA-2a; NB1 glycoprotein; Neutrophil Glycoprotein NB1; RGD1562941; CD177 antigen; Polycythemia rubra vera protein 1; PRV-1; NB1 glycoprotein; NB1 GP; Human neutrophil alloantigen 2a; HNA-2a; CD177; NB1; CD177_MOUSE. |
 |
Specific References (4) | bs-1482R has been referenced in 4 publications.
[IF=3.97] Han, Feifei, et al. "Oral administration of yeast β-glucan ameliorates inflammation and intestinal barrier in dextran sodium sulfate-induced acute colitis." Journal of Functional Foods 35 (2017): 115-126. IHC-P ; Mouse.
[IF=3.73] Scott, Jason A., et al. "The Multifunctional Ca2+/Calmodulin-Dependent Kinase IIdelta (CaMKIIdelta) Regulates Arteriogenesis in a Mouse Model of Flow-Mediated Remodeling." PloS one 8.8 (2013): e71550. IHC-P ; Mouse.
[IF=3.7] Yang, Jian, et al. "Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis." Journal of Cellular and Molecular Medicine (2015). IHC-P ; Rat.
[IF=3.166] Ning YL et al. Caffeine attenuates brain injury but increases mortality induced by high-intensity blast wave exposure. (2019) Toxicology Letters;Feb;301:90-97. IHC ; Mouse.
|
| 研究领域 | 免疫学 细胞表面分子 糖蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 90kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat CD177: 521-650/818 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014] Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). Similarity: Contains 2 UPAR/Ly6 domains. SWISS: D3ZCH9 Gene ID: 499099 Database links: Entrez Gene: 57126 Human Omim: 162860 Human SwissProt: Q8N6Q3 Human Unigene: 232165 Human |
| 产品图片 |
Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 3: Lymph node (Rat) Lysate at 40 ug Primary: Anti-CD177 (bs-1482R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90-120 kD Observed band size: 130 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
