[IF=4.61] Hammond, Jennetta W., Shao-Ming Lu, and Harris A. Gelbard. "Platelet Activating Factor Enhances Synaptic Vesicle Exocytosis Via PKC, Elevated Intracellular Calcium, and Modulation of Synapsin 1 Dynamics and Phosphorylation." Frontiers in cellular neuroscience 9 (2015).  IF(ICC) ;  Rat.  

PubMed:26778968

[IF=4.582] Silvestro Ennio D'Anna. et al. Bacterial load and related innate immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease. RESP MED. 2023 Aug;215:107297  IHC ;  Human.  

PubMed:37245650

[IF=4.174] Jiaqi Jin. et al. Myocardial ischemia-reperfusion injury is probably due to the excessive production of mitochondrial ROS caused by the activation of 5-HT degradation system mediated by PAF receptor. MOL IMMUNOL. 2023 Mar;155:27  WB ;  Rat.  

PubMed:36682136

Sample:
Lane1: Lung(Rat) Lysate at 30 ug
Lane2: Brain(Rat) Lysate at 30 ug
Primary: Anti-PTAFR (bs-1478R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 38kD
Observed band size : 38kD

Sample: Lymph (Mouse) Lysate at 30 ug
Primary: Anti- RAFR/PAF (bs-1478R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD

Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAFR) Polyclonal Antibody, Unconjugated (bs-1478R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated(bs-1478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated(bs-1478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control: 293T cells(blue).
Primary Antibody:Rabbit Anti-PAFR/PAF antibody(bs-1478R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1478R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.