| 产品编号 | bs-1472R |
| 英文名称 | IFI16 Rabbit pAb |
| 中文名称 | γ干扰素诱导蛋白16抗体 |
| 别 名 | gamma-interferon-inducible protein Ifi16; Interferon-inducible myeloid differentiation transcriptional activator; A730048F03; D3; D3cDNA; Ifi-d3; IFI16; IFI16B; IFI204; IFI205; Ifi205a; Ifi205b; IFNGIP1; MGC9466; Mnda; p204; PYHIN2; IFI16; gamma interferon inducible protein 16; gamma-interferon-inducible protein Ifi16; IF16_HUMAN; interferon activated gene 204; Interferon gamma induced protein IFI 16; Interferon gamma inducible protein 16; Interferon-inducible myeloid differentiation transcriptional activator. |
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Specific References (1) | bs-1472R has been referenced in 1 publications.
[IF=1.69] Li et al. Histopathology of melanosis coli and determination of its associated genes by comparative analysis of expression microarrays. (2015) Mol.Med.Re. 12:5807-15 IF,WB ; Human.
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| 研究领域 | 肿瘤 免疫学 发育生物学 信号转导 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 86 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IFI16: 201-350/785 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells. Function: May function as a transcriptional repressor. Could have a role in the regulation of hematopoeitic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in the senescence of prostate epithelial cells. Subcellular Location: Nucleus. Cytoplasm. Uniformly distributed throughout the interphase cell nucleus. Associates with chromatin. Tissue Specificity: Expressed constitutively in cells of the myeloid lineage. Found in promyelocyte stage cells as well as in all other stage cells including peripheral blood monocytes and granulocytes. Also appear in myeloblast cells in some cases of acute myeloid Leukemia. Similarity: Contains 1 DAPIN domain. Contains 1 HIN-200 domain. SWISS: Q16666 Gene ID: 3428 Database links: Entrez Gene: 3428 Human Omim: 147586 Human SwissProt: Q16666 Human Unigene: 380250 Human 信号传导（Signaling Intermediates） |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal go
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
