| 产品编号 | bs-1460R |
| 英文名称 | IL-23R Rabbit pAb |
| 中文名称 | 白介素-23受体抗体 |
| 别 名 | PSORS7; IL-23R; IL23R_HUMAN; IL23R; IL-23 receptor; IL23R_MOUSE; |
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Specific References (3) | bs-1460R has been referenced in 3 publications.
[IF=11.51] Chuang HC et al. GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease. (2018) Sci Adv. Sep 12;4(9):eaat5401. Immunoblotting ; Mouse.
[IF=3.33] Wang, Mingfei, et al. "Damage effect of Interleukin (IL)-23 on oxygen-glucose deprived cells of the neurovascular unit via IL-23 receptor." Neuroscience(2015). IHC-P ; Mouse.
[IF=3.234] Wang H et al. NLRP3 inflammasome activates interleukin-23/interleukin-17 axis during ischaemia-reperfusion injury in cerebral ischaemia in mice. Life Sci. 2019 Jun 15;227:101-113. WB ; Mouse.
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| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-1000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 67 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-23R: 401-550/629 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. Function: Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Subunit: Heterodimer with IL12RB1. In presence of IL23, the heterodimer forms the IL23 receptor. Interacts with JAK2 and in presence of IL23 with STAT3. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells. Post-translational modifications: Phosphorylated in response to IL23. DISEASE: Genetic variations in IL23R are associated with inflammatory bowel disease type 17 (IBD17) [MIM:612261]. IBD17 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Similarity: Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 2 fibronectin type-III domains. SWISS: Q5VWK5 Gene ID: 149233 Database links: Entrez Gene: 149233 Human Entrez Gene: 209590 Mouse Omim: 607562 Human SwissProt: Q5VWK5 Human SwissProt: Q8K4B4 Mouse Unigene: 677426 Human Unigene: 221227 Mouse |
| 产品图片 |
Sample:
Lane 1: Human K562 cell lysates
Lane 2: Human Jurkat cell lysates
Primary: Anti-IL-23R (bs-1460R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 68 kDa
Sample:
Lane 1: Lymph node (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Rat) Lysate at 40 ug
Lane 3: Thymus (Rat) Lysate at 40 ug
Primary: Anti-IL-23R (bs-1460R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 75 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
