| 产品编号 | bs-1445R |
| 英文名称 | Rabbit Anti-Motilin antibody |
| 中文名称 | 胃动素抗体 |
| 别 名 | Motilin; MGC138519; MLN; Motilin-associated peptide; Promotilin; MOTI_HUMAN. |
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Specific References (2) | bs-1445R has been referenced in 2 publications.
[IF=10.257] Jiao Ma. et al. A robust composite hydrogel consisting of polypyrrole and β-cyclodextrin-based supramolecular complex for the label-free amperometric immunodetection of motilin with well-defined dual signal response and high sensitivity. Biosens Bioelectron. 2021 Feb;173:112810 Other ;
[IF=1.52] Liang Q et al. Evaluation of a modified rat model for functional dyspepsia.Saudi J Gastroenterol. 2018 Jul-Aug;24(4):228-235. IHC-P ; Rat.
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| 研究领域 | 细胞生物 神经生物学 内分泌病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Rabbit,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 10.3kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MLN: 51-115/115 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008] Function: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Subcellular Location: Secreted. Similarity: Belongs to the motilin family. SWISS: P12872 Gene ID: 4295 Database links: Entrez Gene: 4295 Human Omim: 158270 Human SwissProt: P12872 Human SwissProt: P27114 Rabbit SwissProt: O18845 Sheep Unigene: 2813 Human 胃动素属于兴奋胃肠道平滑肌运动的脑肠肽，主要在胃肠道表达。 |
| 产品图片 |
Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Motilin Polyclonal Antibody, Unconjugated(bs-1445R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
