| 产品编号 | bs-0930R |
| 英文名称 | phospho-heterochromatin protein 1 (pTyr41) |
| 中文名称 | 异染色质蛋白1磷酸化抗体（果蝇） |
| 别 名 | Antigen p25; Cbx 5; Cbx5 antibody Chromobox homolog 5 (Drosophila HP1 alpha); Chromobox homolog 5 (HP1 alpha homolog Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Chromobox protein homologue 5 ; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP 1; HP 1A; HP1 alpha homolog ; HP1A ; HP1Hs alpha; HP1Hs-alpha. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 昆虫 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Fruit Fly) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23kDa |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human SLC7A11around the phosphorylation site of Ser481: VE(p-Y)YL |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin. SWISS: P05205 Gene ID: N/A Database links: GenBank: ACI96782.1 异染色质蛋白1（Heterochromatin protein 1 (HP1)）一般认为是异染色质的维持者，异染色质是转录沉默的固缩DNA，常常与中心粒等区域有关。这种固缩的DNA被认为是转录因子无法接近的，但近期的Science上发表的两篇报道对这个观点提出了挑战。 这个新的研究提出HP1可能是异染色质活性调控的重要因子，在细胞生理活动中具有的重要功能。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
