| 产品编号 | bs-0928R |
| 英文名称 | Rabbit Anti-phospho-heterochromatin protein 1 (pTyr24) antibody |
| 中文名称 | 磷酸化异染色质蛋白1抗体（果蝇） |
| 别 名 | heterochromatin protein 1(phospho Tyr24); heterochromatin protein 1(phospho Y24); Antigen p25; Cbx 5; Cbx5; Chromobox homolog 5(Drosophila HP1 alpha); Chromobox homolog 5(HP1 alpha homolog Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Chromobox protein homologue 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP 1; HP 1A; HP1 alpha homolog; HP1A; HP1Hs alpha; HP1Hs-alpha. |
 |
Specific References (1) | bs-0928R has been referenced in 1 publications.
[IF=3.057] Terzuoli et al. Antagonism of bradykinin B2 receptor prevents inflammatory responses in human endothelial cells by quenching the NF-kB pathway activation. (2014) PLoS.On. 9:e84358 WB ; Human.
|
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 免疫学 染色质和核信号 昆虫 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Fruit Fly) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from Fruit Fly HP1 around the phosphorylation site of Tyr24: EE(p-Y)AV |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane.
HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required
for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin. Database links: GenBank: ACI96782.1 异染色质蛋白1（Heterochromatin protein 1 (HP1)）一般认为是异染色质的维持者，异染色质是转录沉默的固缩DNA，常常与中心粒等区域有关。这种固缩的DNA被认为是转录因子无法接近的，但近期的Science上发表的两篇报道对这个观点提出了挑战。 这个新的研究提出HP1可能是异染色质活性调控的重要因子，在细胞生理活动中具有的重要功能。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
