| 产品编号 | bs-1441R |
| 英文名称 | Rabbit Anti-CXCL16 antibody |
| 中文名称 | CXC趋化因子16抗体 |
| 别 名 | CXC-chemokine ligand 16; Chemokine(C X C motif) ligand 16; CXC chemokine ligand 16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small inducible cytokine B16 precursor; SRPSOX; Transmembrane chemokine CXCL16; CXL16_HUMAN. |
 |
Specific References (5) | bs-1441R has been referenced in 5 publications.
[IF=8.063] Hu ZB et al. Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis. Theranostics. 2020 Feb 3;10(6):2803-2816. IHC,WB,IF ; rat.
[IF=6.68] Cottle, Denny L., et al. "Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis." Human molecular genetics (2014): ddu459. Mouse.
[IF=6.291] Changjiang Liu. et al. JNK and Jag1/Notch2 co-regulate CXCL16 to facilitate cypermethrin-induced kidney damage. ECOTOX ENVIRON SAFE. 2022 Jun;238:113582 WB,IF ; Rat.
[IF=3.324] Ma et al. CXCL16 regulates renal injury and fibrosis in experimental renal artery stenosis. (2016) Am.J.Physiol.Heart.Circ.Physio. 311:H815-21 IF(IHC-F) ; Rat.
[IF=2.447] Yanji Zhu. et al. P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy. Exp Ther Med. 2022 Feb;23(2):1-8 IHC ; Mouse.
|
| 研究领域 | 肿瘤 免疫学 信号转导 转录调节因子 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of human CXCL16: 85-200/247 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. It induces a strong chemotactic response and calcium mobilization. It binds to CXCR6/Bonzo. Subcellular Location: Cell membrane. Secreted. Also exists as a soluble form. Tissue Specificity: Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow. Similarity: Belongs to the intercrine alpha (chemokine CxC) family. SWISS: Q9H2A7 Gene ID: 58191 Database links: Entrez Gene: 58191 Human Omim: 605398 Human SwissProt: Q9H2A7 Human Unigene: 513885 Human |
| 产品图片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CXCL16 Polyclonal Antibody, Unconjugated(bs-1441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (bs-1441R) at 1:50 for two hours at room temperature (green channel).
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (bs-1441R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
