| 产品编号 | bs-1436R |
| 英文名称 | PBP Rabbit pAb |
| 中文名称 | 磷脂酰乙醇胺结合蛋白1抗体 |
| 别 名 | phosphatidylethanolamine-binding protein; PEBP; PEBP 1; PBP; RKIP; Phosphatidylethanolamine-binding protein 1; PEBP-1; PEBP1; Prostatic-binding protein; Prostatic binding protein; Neuropolypeptide h3; HCNPpp; Raf kinase inhibitor protein; Hippocampal cholinergic neurostimulating peptide; HCNP. |
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Specific References (2) | bs-1436R has been referenced in 2 publications.
[IF=1.581] Ping et al. Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations. (2015) Int.J.Clin.Exp.Pathol. 8:10164-70 IHC ; Human.
[IF=1.39] Wei et al. Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma. (2017) Oncol.Lett. 13:1866-1872 IHC-P ; Human.
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| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 细胞周期蛋白 转录调节因子 激酶和磷酸酶 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RKIP: 51-187/187 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. [SUBCELLULAR LOCATION] Cytoplasm. Belongs to the phosphatidylethanolamine-binding protein family. Function: Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. Subcellular Location: Cytoplasm. Similarity: Belongs to the phosphatidylethanolamine-binding protein family. SWISS: P30086 Gene ID: 5037 Database links: Entrez Gene: 5037 Human Entrez Gene: 23980 Mouse Omim: 604591 Human SwissProt: P30086 Human SwissProt: P70296 Mouse Unigene: 433863 Human Unigene: 195898 Mouse Unigene: 338476 Mouse Unigene: 473809 Mouse Unigene: 29745 Rat 信号传导（Signaling Intermediates） Raf激酶抑制蛋白(PKIP)属于磷脂酰乙醇胺结合蛋白 （phosphatidylethanolamine-binding protein, PEBP）家族,广泛存在于各种不同的生物中,PKIP还参与了对NF-kB信号通路和G蛋白偶联受体信号通路的调控,参与NF-κB信号通路的调控,参与了对细胞内多种信号转导通路的调节作用。RKIP在膜的生物合成、精子发生、神经发育和细胞凋亡等生理过程中发挥重要作用，并参与了老年痴呆症及糖尿病等的病理过程。有学者认为：RKIP有抑制肿瘤细胞转移的作用。 |
| 产品图片 |
Sample: Brain(mouse) at lysates;
Primary: Anti-PBP (bs-1436R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:21 kD
Observed band size:21 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RKIP/PBP Polyclonal Antibody, Unconjugated(bs-1436R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
