产品中心-北京博奥森生物技术有限公司

产品编号	bs-1396R
英文名称	CEBP Beta
中文名称	转录调节因子C/EBP β抗体
别名	CEBP-beta; CLEBP beta; CCAAT Enhancer Binding Protein beta; AGP/EBP; C EBP beta; C/EBP related protein 2; CEBPB; CRP2; IL 6DBP; IL6DBP; Interleukin 6 dependent binding protein; LAP; Liver enriched transcriptional activator; NF IL6; NFIL6; Nuclear factor NF IL6; SF B; SFB; Silencer factor B; TCF5; Transcription factor 5; C/EBP beta; CCAAT/enhancer-binding protein beta; CEBPB_HUMAN; Liver activator protein; Liver enriched transcriptional activator; Nuclear factor NF IL6; Nuclear factor NF-IL6; TCF-5.
	Specific References (12) \| bs-1396R has been referenced in 12 publications. [IF=6.175] Li Kan. et al. m6A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte. Journal of Animal Science and Biotechnology. 2022 Dec;13(1):1-15 WB ; Chicken. PubMed:36461116 [IF=5.62] He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015). WB ; Human. PubMed:26272182 [IF=4.575] Ren, Teng-Teng. et al. Gisenoside Rg1 attenuates cadmium-induced neurotoxicity in vitro and in vivo by attenuating oxidative stress and inflammation. Inflamm Res. 2021 Dec;70(10):1151-1164 WB ; Mouse. PubMed:34661679 [IF=4.357] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human. PubMed:26538209 [IF=4.259] Wu Z et al. Co-infection of Mycoplasma gallisepticum and Escherichia coli Triggers Inflammatory Injury Involving the IL-17 Signaling Pathway. Front Microbiol. 2019 Nov 15;10:2615. WB ; Chicken. PubMed:31803158 [IF=3.231] Pan-Pan Guo. et al. Overexpression of DGAT2 Regulates the Differentiation of Bovine Preadipocytes. ANIMALS. 2023 Jan;13(7):1195 WB ; Bovine. PubMed:37048451 [IF=3.105] Huan-Tong Wu. et al. Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice. Neurotoxicology. 2021 Jul;85:68 WB ; Rat. PubMed:34004234 [IF=2.02] Jiejuan Lai. et al. Comparison of the biological and functional characteristics of mesenchymal stem cells from intrahepatic and identical bone marrow. Stem Cell Res. 2021 Aug;55:102477 IHC ; Mouse. PubMed:34343826 [IF=1.922] Wang F et al. Insulin‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Mol Med Rep. 2019 Jan 24. WB ; Human. PubMed:30720075 [IF=1.88] Shihe Zhang. et al. Astragalus polysaccharide regulates brown adipocytes differentiation by miR-6911 targeting Prdm16. 2021 Nov 05 WB ; Mouse. PubMed:34738642 [IF=1.832] Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193. WB ; ram lambs. PubMed:31022943 [IF=1.448] Zhang K et al. Insulin‑like growth factor 2 promotes the adipogenesis of hemangioma‑derived stem cells. Exp Ther Med. 2019 Mar;17(3):1663-1669. WB ; Human. PubMed:30867686
研究领域	肿瘤染色质和核信号转录调节因子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse (predicted: Rat,Chicken,Pig,Cow,Rabbit,Sheep)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	36kDa
细胞定位	细胞核
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human CEBP Beta: 251-345/345
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq]. Function: Involved in interleukin-6 signal transduction, including the transcriptional activation of acute-phase genes. Functions in brown adipose tissue (BAT) differentiation. Subunit: Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Subcellular Location: Nucleus. Post-translational modifications: Sumoylated by polymeric chains of SUMO2 or SUMO3. Similarity: Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain. SWISS: P17676 Gene ID: 1051 Database links: Entrez Gene: 1051 Human Entrez Gene: 12608 Mouse Entrez Gene: 24253 Rat Omim: 189965 Human SwissProt: P17676 Human SwissProt: P28033 Mouse SwissProt: P21272 Rat Unigene: 517106 Human Unigene: 719041 Human Unigene: 720603 Human Unigene: 439656 Mouse Unigene: 6479 Rat 转录调节因子（Transcriptin Regulators）
产品图片	Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-CEBP Beta (bs-1396R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 41 kD Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-CEBP Beta (bs-1396R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: MG63(Human) Cell Lysate at 40 ug Primary: Anti-CEBP Beta (bs-1396R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: HepG2(Human) Cell Lysate at 40 ug Primary: Anti-CEBP Beta (bs-1396R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBP Beta) Polyclonal Antibody, Unconjugated (bs-1396R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela Primary Antibody (green line): Rabbit Anti-CEBP Beta antibody (bs-1396R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记