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Rabbit Anti-Beclin 1  antibody (bs-1353R)  
~~~促销代码KT202411~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1353R
英文名称 Rabbit Anti-Beclin 1  antibody
中文名称 自噬效应蛋白Beclin 1抗体
别    名 Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.  
Specific References  (44)     |     bs-1353R has been referenced in 44 publications.
[IF=16.016] Jilong Zhou. et al. ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation. 2022 Mar 20  WB ;  Mouse.  
[IF=16.016] Hainan He. et al. Selective autophagic degradation of ACLY (ATP citrate lyase) maintains citrate homeostasis and promotes oocyte maturation. AUTOPHAGY. 2022 Apr 25  WB ;  Pig.  
[IF=10.171] Wan Zhou. et al. Retinol binding protein 4 promotes the phenotypic transformation of vascular smooth muscle cells under high glucose condition via modulating RhoA/ROCK1 pathway. TRANSL RES. 2023 Mar;:  WB ;  Rat.  
[IF=9.988] Yue Zhang. et al. Endoplasmic reticulum stress-controlled autophagic pathway promotes polystyrene microplastics-induced myocardial dysplasia in birds. ENVIRON POLLUT. 2022 Oct;311:119963  WB ;  Chicken.  
[IF=7.31] Yi-Jing Yang. et al. Lycium barbarum Polysaccharides Regulating miR-181/Bcl-2 Decreased Autophagy of Retinal Pigment Epithelium with Oxidative Stress. OXID MED CELL LONGEV. 2023 Jan 05;2023:9554457  WB ;  Mouse.  
[IF=7.086] Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625  IF ;  Mouse.  
[IF=5.923] Hyoung Moon Kim. et al. Evaluating Whether Radiofrequency Irradiation Attenuated UV-B-Induced Skin Pigmentation by Increasing Melanosomal Autophagy and Decreasing Melanin Synthesis. Int J Mol Sci. 2021 Jan;22(19):10724  WB ;  Mouse.  
[IF=5.811] Lang Xiu-Yuan. et al. Coeloglossum viride Var. Bracteatum Extract Attenuates MPTP-Induced Neurotoxicity in vivo by Restoring BDNF-TrkB and FGF2-Akt Signaling Axis and Inhibiting RIP1-Driven Inflammation. FRONT PHARMACOL. 2022 Apr;0:1571  WB ;  Mouse.  
[IF=5.81] Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784  WB,IHC ;  Mouse.  
[IF=5.75] Liu P. et al. Scalp Acupuncture Attenuates Brain Damage After Intracerebral Hemorrhage Through Enhanced Mitophagy and Reduced Apoptosis in Rats.. Front Aging Neurosci. 2021 Dec;13:718631-718631  WB,IHC ;  Rat.  
[IF=5.714] Tengyun Yang. et al. MicroRNA-146a-5p alleviates the pathogenesis of osteoarthritis by inhibiting SDF-1/CXCR4-induced chondrocyte autophagy. INT IMMUNOPHARMACOL. 2023 Apr;117:109938  WB ;  Rabbit.  
[IF=5.64] Chengcheng Zhang. et al. Autophagy Induced by the N-Terminus of the Classic Swine Fever Virus Nonstructural Protein 5A Protein Promotes Viral Replication. Front Microbiol. 2021; 12: 733385  WB ;  Pig.  
[IF=5.581] Ibitamuno Caleb. et al. The Effects of Rapamycin on the Intestinal Graft in a Rat Model of Cold Ischemia Perfusion and Preservation. METABOLITES. 2022 Sep;12(9):794  IHC ;  Rat.  
[IF=5.23] Chen, Cheng-Hsien, et al. "Far-infrared protects vascular endothelial cells from advanced glycation end products-induced injury via PLZF-mediated autophagy in diabetic mice." Scientific Reports 7 (2017): 40442.  IHC-P ;  Mouse.  
[IF=5.195] Yun Shi. et al. Protection of Taohong Siwu Decoction on PC12 cells injured by oxygen glucose deprivation/reperfusion via mitophagy-NLRP3 inflammasome pathway in vitro. J ETHNOPHARMACOL. 2022 Oct;:115784  IF ;  Rat.  
[IF=5.194] Yu-Shen Luo. et al. Acrylonitrile exposure triggers ovarian inflammation and decreases oocyte quality probably via mitochondrial dysfunction induced apoptosis in mice. CHEM-BIOL INTERACT. 2022 Jun;360:109934  WB ;  Mouse.  
[IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801  WB ;  Human.  
[IF=4.939] Tong-Fei Li. et al. Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma. Mol Pharmaceut. 2021;XXXX(XXX):XXX-XXX  WB ;  Pig.  
[IF=4.932] Ibitamuno Caleb. et al. Characterizing Autophagy in the Cold Ischemic Injury of Small Bowel Grafts: Evidence from Rat Jejunum. Metabolites. 2021 Jun;11(6):396  IHC ;  Rat.  
[IF=4.432] Wei Jiang. et al. Caveolin-1 attenuates acetaminophen aggravated lipid accumulation in alcoholic fatty liver by activating mitophagy via the Pink-1/Parkin pathway. Eur J Pharmacol. 2021 Oct;908:174324  WB ;  Mouse, Human.  
[IF=4.432] Jinni Meng. et al. Dehydrocostuslactone attenuated oxygen and glucose deprivation/reperfusion-induced PC12 cell injury through inhibition of apoptosis and autophagy by activating the PI3K/AKT/mTOR pathway. Eur J Pharmacol. 2021 Nov;911:174554  WB ;  Rat.  
[IF=4.414] Liu, Zhan. et al. Kaemperfol Protects Dopaminergic Neurons by Promoting mTOR-Mediated Autophagy in Parkinson’s Disease Models. NEUROCHEM RES. 2022 Dec;:1-17  IHC, WB ;  Mouse, Rat.  
[IF=4.3] Wang, Jinheng, et al. "Bcl-3, induced by Tax and HTLV-1, inhibits NF-κB activation and promotes autophagy." Cellular Signalling (2013).  WB ;  Human.  
[IF=4.236] Qi-Rui Li. et al. Laser-triggered intelligent drug delivery and anti-cancer photodynamic therapy using platelets as the vehicle. PLATELETS. 2023;34(1):Article: 2166677  WB ;  Mouse.  
[IF=4.2] Yang, Huan, et al. "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." Stem Cell Research & Therapy 8.1 (2017): 267.  WB ;  Human.  
[IF=3.776] Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4α signaling in HLSECs. HELIYON. 2022 Dec;8:e12385  WB ;  Human.  
[IF=3.414] Ma HX et al. Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway. J Ethnopharmacol. 2020 Jan 22;252:112583.  ICF&WB ;  Rattus norvegicus.  
[IF=3.263] Fengge Shen. et al. Aloe-emodin induces autophagy and apoptotic cell death in non-small cell lung cancer cells via Akt/mTOR and MAPK signaling. Eur J Pharmacol. 2020 Nov;886:173550  WB ;  Human.  
[IF=3.23] Sun, Qianqian, et al. "Factors that Affect Pancreatic Islet Cell Autophagy in Adult Rats: Evaluation of a Calorie-Restricted Diet and a High-Fat Diet."PLOS ONE 11.3 (2016): e0151104.  IHC-P ;  Rat.  
[IF=3.216] Lijuan Gao # et al. Chlorogenic Acid Alleviates Aβ25-35-Induced Autophagy and Cognitive Impairment via the mTOR/TFEB Signaling Pathway. Drug Des Devel Ther. 2020 May 4;14:1705-1716.  WB ;  Human.  
研究领域 肿瘤  免疫学  神经生物学  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat,Pig
产品应用 WB=1:500-2000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 50kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BECN1: 201-330/450 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.

Function:
Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Subunit:
Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1.

Subcellular Location:
Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.
Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.

Similarity:
Belongs to the beclin family.

SWISS:
Q14457

Gene ID:
8678

Database links:

Entrez Gene: 8678 Human

Entrez Gene: 56208 Mouse

Entrez Gene: 114558 Rat

Omim: 604378 Human

SwissProt: Q14457 Human

SwissProt: O88597 Mouse

SwissProt: Q91XJ1 Rat

Unigene: 716464 Human

Unigene: 178947 Mouse

Unigene: 2776 Rat



BECN1是一种分子质量为60KDa的蛋白质, 它能与细胞凋亡抑制剂Bcl-2相互作用, 减少中枢神经系统的凋亡,参与PI3K复合物的组成和自噬体的形成。主要表达在胞质。抑癌基因beclin 1通过自噬作用来调节细胞生长。
产品图片
Sample: Pig Primary: Anti-Beclin-1 (bs-1353R) at 1:500 dilution;
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-1353R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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