| 产品编号 | bs-1318R |
| 英文名称 | IL-5 Rabbit pAb |
| 中文名称 | 白细胞介素5抗体 |
| 别 名 | B Cell Differentiation Factor I; IL5; IL 5; Colony stimulating factor; Colony Stimulating Factor Eosinophil; EDF; Eosinophil; Eosinophil Differentiation Factor; IL 5; Interleukin 5; B-cell differentiation factor I; IL-5; IL5_RAT; Interleukin-5; T Cell Replacing Factor; T-cell replacing factor; Interleukin5; TRF. |
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Specific References (1) | bs-1318R has been referenced in 1 publications.
[IF=13.081] Xu Jun Yan. et al. Interleukin-5-induced eosinophil population improves cardiac function after myocardial infarction. CARDIOVASC RES. 2022 Jul;118(9):2165-2178 WB ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse IL-5: 56-132/132 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Interleukin 5 is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology. Function: Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted. Similarity: Belongs to the IL-5 family. Database links: Entrez Gene: 3567 Human Omim: 147850 Human SwissProt: P05113 Human Unigene: 2247 Human 白细胞介素-5（IL-5）的表达对嗜酸粒细胞（EOS）有浸润聚集的作用，目前多用变态反应方面的研究。 支气管哮喘的重要特征主要是慢性非特异性炎症改变，其病因与发病机制十分复杂，白介素5在支气管哮喘发病机理上，近年来受到了很大关注。 |
| 产品图片 |
Sample:
Lovo Cell (Human) Lysate at 30 ug
Primary: Anti- IL-5 (bs-1318R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13kD
Observed band size: 10kD
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (bs-1318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (bs-1318R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (bs-1318R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
