| 产品编号 | bs-0917R |
| 英文名称 | BrdU(Proliferation Marker) |
| 中文名称 | 5-溴脱氧尿嘧啶核苷（增殖标志物）抗体 |
| 别 名 | Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine. |
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Specific References (3) | bs-0917R has been referenced in 3 publications.
[IF=6.656] Wei Hao. et al. Classical prescription Huanglian Decoction relieves ulcerative colitis via maintaining intestinal barrier integrity and modulating gut microbiota. PHYTOMEDICINE. 2022 Sep;:154468 IF ; Mouse.
[IF=5.195] Lu Hong. et al. Investigation of Naoluoxintong on the neural stem cells by facilitating proliferation and differentiation in vitro and on protecting neurons by up-regulating the expression of nestin in MCAO rats. J ETHNOPHARMACOL. 2022 Sep;:115684 IF ; Rat.
[IF=3.659] Wang, Xiao-feng. et al. Active constituent of Polygala tenuifolia attenuates cognitive deficits by rescuing hippocampal neurogenesis in APP/PS1 transgenic mice. Bmc Complem Altern M. 2021 Dec;21(1):1-15 IF ; Mouse.
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| 产品类型 | 小分子抗体 |
| 研究领域 | 细胞生物 发育生物学 染色质和核信号 神经生物学 细胞凋亡 细胞周期蛋白 药物及化合物 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: BrdU) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 0.3071kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Brdu |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985). Subcellular Location: Nuclear. SWISS: N/A CAS: 59-14-3 BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。 用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。 |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Brdu Polyclonal Antibody, Unconjugated(bs-0917R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
