| 产品编号 | bs-0922R |
| 英文名称 | Neurogenin3 Rabbit pAb |
| 中文名称 | 神经元素3抗体 |
| 别 名 | NGN3; NGN-3; neurogenin 3; Neurog3; Neurogenin-3; ATH4B; Atoh5; Math4B; NEUROG3; ngn3; OTTHUMP00000019730; Protein atonal homolog 5; Class A basic helix-loop-helix protein 7; NGN3_HUMAN. 神经营养素-3; |
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Specific References (2) | bs-0922R has been referenced in 2 publications.
[IF=2.08] Jian, Ruo-Lei, et al. "Generation of insulin-producing cells from C3H10T1/2 mesenchymal progenitor cells." Gene (2015). WB ; Mouse.
[IF=0] Wenhuan Chai. et al. Visualizing Cathepsin K-Cre Expression at the Single-Cell Level with GFP Reporters. JBMR Plus. 2022 Dec;:e10706 IF ; Mouse.
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| 研究领域 | 细胞生物 神经生物学 干细胞 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NGN3: 61-160/214 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm. Function: Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Subcellular Location: Nucleus. DISEASE: Defects in NEUROG3 are the cause of diarrhea type 4 (DIAR4) [MIM:610370]. DIAR4 is a characterized by severe, life-threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: Q9Y4Z2 Gene ID: 50674 Database links: Entrez Gene: 50674 Human Entrez Gene: 11925 Mouse Omim: 604882 Human SwissProt: Q9Y4Z2 Human SwissProt: P70661 Mouse Unigene: 532682 Human Unigene: 57236 Mouse 神经生物学相关蛋白（Neurobiology） Ngn3属于bHLH家族蛋白，是胰腺发育过程中短暂表达的蛋白，可能是胰腺内分泌细胞系的定向因子，一般在成熟胰岛中不存在。 Ngn3的表达会导致胰腺内分泌细胞数的增加外分泌细胞数的减少。因而胰岛β细胞并不是完全靠干细胞分化产生，主要是通过已分化胰岛β细胞的分裂增殖。 但近年来，有学者认为：Ngn3虽在胰腺发育中短暂表达，但是否存在使胰岛干细胞或前体细胞自我更新的能力尚未明确定论。 |
| 产品图片 |
Sample:Liver(Mouse) Lysate at 30 ug
Primary: Anti-Neurogenin3 (bs-0922R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 26 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
