| 产品编号 | bs-1315R |
| 英文名称 | Nogo B |
| 中文名称 | 轴索过度生长抑制因子B抗体 |
| 别 名 | reticulon-4 isoform B; RTN4; RTN4-B1; Nbla10545; RTN-X; reticulon-4A; Nogo-C; NSP; RTN-x; ASY; foocen; NSP-CL; NOGOC; RTN4-A; Reticulon-4; Nbla00271; KIAA0886; NOGOA; Reticulon-5; Foocen; NI220/250; RTN4-C; RTN4-B2; SP1507; NOGO-A; NOGOB; Nogo-B; Nogo-B/A. |
| 研究领域 | 神经生物学 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RTN4 isoform B: 251-357/357 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. Function: NOGO is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Adult mammalian axon regeneration is generally successful in the peripheral nervous system but poor in the central nervous system. Inhibition results from physical barriers imposed by glial scars, a lack of neurotrophic factors, and growth-inhibitory molecules associated with myelin, the insulating axon sheath. These molecules include NI35, myelin-associated glycoprotein (159460), and Nogo. Several isoforms (A-E) of NOGO exist. Subcellular Location: Endoplasmic reticulum; endoplasmic reticulum membrane; multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. SWISS: Q9NQC3 Gene ID: 57142 Database links: Entrez Gene: 57142 Human Entrez Gene: 68585 Mouse Omim: 604475 Human SwissProt: Q9NQC3 Human SwissProt: Q99P72 Mouse Unigene: 704007 Human 神经生物学相关蛋白（Neurobiology） Nogo-B蛋白是一组参与组织损伤后再生的跨膜功能蛋白，其中Nogo-B位于多种组织器官中而发挥不同的功能。目前研究发现，Nogo-B与肿瘤抑制、凋亡、神经系统再生抑制、血管内膜损伤修复等多种病理生理过程有不同程度的关系. |
| 产品图片 |
Sample:
Muscle (Mouse) Lysate at 40 ug Primary: Anti-Nogo A+B (bs-1315R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD 
Sample:
Lane 1: Cerebellum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-Nogo B (bs-1315R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 45 kD 
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nogo-B Polyclonal Antibody, Unconjugated(bs-1315R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
