| 产品编号 | bs-1294R |
| 英文名称 | Rabbit Anti-ODC1 antibody |
| 中文名称 | 鸟氨酸脱羧酶抗体 |
| 别 名 | Ornithine decarboxylase 1; Ornithine decarboxylase; Dodc1; Odc 1; ODC1; ODC2; Ornithine decarboxylase 2; DCOR_HUMAN; Ornithine decarboxylase 1; Ornithine decarboxylase; Ornithine decarboxylase structural 1; RNODC. |
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Specific References (5) | bs-1294R has been referenced in 5 publications.
[IF=4.492] Jing Wanget al. Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation. Anim Nutr
. 2020 Dec;6(4):438-446. WB ; pig.
[IF=3.789] Wang, Hongliang. et al. A new 68Ga-labeled ornithine derivative for PET imaging of ornithine metabolism in tumors. AMINO ACIDS. 2023 Feb;:1-12 IHC ; Mouse.
[IF=2.83] Chen, Shaokui, et al. "Asparagine improves intestinal integrity, inhibits TLR4 and NOD signaling, and differently regulates p38 and ERK1/2 signaling in weanling piglets after LPS challenge." Innate Immunity (2016): 1753425916664124. WB ; Pig.
[IF=2.48] Rossi et al. Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer. (2015) Biomed.Res.In. 2015:172756 IHC ; dog.
[IF=1.58] Rossi, Giacomo, et al. "Immunohistochemical Expression of Ornithine Decarboxylase, Diamine Oxidase, Putrescine, and Spermine in Normal Canine Enterocolic Mucosa, in Chronic Colitis, and in Colorectal Cancer." BioMed Research International 2015 (2015). IHC-P ; Dog.
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| 研究领域 | 肿瘤 信号转导 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ODC1: 321-461/461 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Ornithine Decarboxylase (ODC) is the initial and rate limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa. Subunit: Homodimer. Post-translational modifications: S-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues. Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family. SWISS: P11926 Gene ID: 4953 Database links: Entrez Gene: 4953 Human Entrez Gene: 18263 Mouse Omim: 165640 Human SwissProt: P11926 Human SwissProt: P00860 Mouse Unigene: 7133 Cow Unigene: 467701 Human Unigene: 34102 Mouse Unigene: 874 Rat 鸟氨酸脱羧酶为多胺生物合成途径中的第一个限速酶 (催化合成多胺的第一步反应 ,即催化L -鸟氨酸脱羧生成腐胺 ) ,具有超常转变率和对于刺激的迅速反应性。 多胺广泛存在于生物体的各种细胞和组织中 ,是一种重要的代谢调控物质 ,它可直接与DNA相互作用 ,调节DNA构象 ,并参与RNA及蛋白质的合成 ,与细胞增殖、分化密切相关。经研究表明 ,哺乳动物细胞增殖体现出完全的多胺依赖性。ODC主要存在于胞浆中。 |
| 产品图片 |
Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD
Sample:Liver(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: AP conjugated Goat Anti-Rabbit IgG(bs-0295G-AP) at 1: 5000 dilution;
Predicted band size : 51kD
Observed band size : 51kD
Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (bs-1294R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (bs-1294R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
