| 产品编号 | bs-1290R |
| 英文名称 | CCN1 Rabbit pAb |
| 中文名称 | 富半胱氨酸肝素结合蛋白61抗体 |
| 别 名 | Cyr61; IGFBP10; cysteine rich protein 61; 3CH61; CCN 1; CCN1; CYR 61; CYR61; CYR61 protein; CYR61 protein precursor; Cysteine rich angiogenic inducer 61; Cysteine rich heparin binding protein 61; GIG 1; GIG1; GIG1; Igfbp 10; IGFBP10; Insulin like growth factor binding protein 10; Protein CYR61; Protein GIG1; AI325051; cysteine rich 61; CYR61_HUMAN . |
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Specific References (2) | bs-1290R has been referenced in 2 publications.
[IF=2.014] Fang-Fang CHEN. et al. Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles. Chin J Nat Medicines. 2020 Aug;18:620 WB ; Rat.
[IF=0.786] Dong QQ et al. SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells. Food Sci Biotechnol. 2018 Mar 22;27(4):1165-1173. WB ; Human.
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| 研究领域 | 肿瘤 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyr61: 251-379/379 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011] Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Subcellular Location: Secreted. Similarity: Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain. SWISS: O00622 Gene ID: 3491 Database links: Entrez Gene: 3491 Human Entrez Gene: 16007 Mouse Omim: 602369 Human SwissProt: O00622 Human SwissProt: P18406 Mouse Unigene: 8867 Human Unigene: 1231 Mouse Unigene: 22129 Rat 高半胱氨酸蛋白61是富含半胱氨酸的分泌性肝素连接蛋白，是CCN家族成员之一，属于生长因子诱导的即刻早期基因，有明显的促有丝分裂和趋化活性作用，有诱导成纤维细胞增殖和分泌细胞外基质等功能，可促进细胞增殖、迁移、黏附、分化和细胞外基质（ECM）合成的作用，在新生血管及肿瘤形成过程中起着重要作用。 |
| 产品图片 |
Sample: U251 Cell Lysate at 40 ug
Primary: Anti-CCN1 (bs-1290R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 48 kD
Sample:
Lane 1: Human Huvec cell lysates
Lane 2: Human A431 cell lysates
Primary: Anti-CCN1 (bs-1290R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti- CCN1 (bs-1290R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCN1) Polyclonal Antibody, Unconjugated (bs-1290R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
