| 产品编号 | bs-0813R |
| 英文名称 | Rabbit Anti-Alpha casein antibody |
| 中文名称 | 酪蛋白抗体 |
| 别 名 | Casein; alpha-S1-casein; CASA; CASA1_Cow; Casein alpha s1; casein, alpha; casein, alpha-S1, included; Casoxin-D; CSN1; CSN1S1; MGC149368. |
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Specific References (20) | bs-0813R has been referenced in 20 publications.
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| 研究领域 | 细胞生物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Cow,Bovine (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Casein from bovine milk |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Casein is the name for a family of related phosphoproteins (αS1, αS2, β, κ). These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive, to a binder for safety matches. As a food source, casein supplies amino acids; carbohydrates; and two inorganic elements, calcium and phosphorus. Subunit: Casein contains a fairly high number of proline residues, which do not interact. There are also no disulfide bridges. As a result, it has relatively little tertiary structure. It is relatively hydrophobic, making it poorly soluble in water. It is found in milk as a suspension of particles called "casein micelles" which show only limited resemblance with surfactant-type micellae in a sense that the hydrophilic parts reside at the surface and they are spherical. However, in sharp contrast to surfactant micelles, the interior of a casein micelle is highly hydrated. The caseins in the micelles are held together by calcium ions and hydrophobic interactions. Several models account for the special conformation of casein in the micelles. One of them proposes the micellar nucleus is formed by several submicelles, the periphery consisting of microvellosities of κ-casein. Another model suggests the nucleus is formed by casein-interlinked fibrils. Finally, the most recent model proposes a double link among the caseins for gelling to take place. All three models consider micelles as colloidal particles formed by casein aggregates wrapped up in soluble κ-casein molecules. The isoelectric point of casein is 4.6. Since milk's pH is 6.6, casein has a negative charge in milk. The purified protein is water insoluble. While it is also insoluble in neutral salt solutions, it is readily dispersible in dilute alkalis and in salt solutions such as sodium oxalate and sodium acetate. The enzyme trypsin can hydrolyze off a phosphate-containing peptone. It is used to form a type of organic adhesive. SWISS: N/A Gene ID: 282208 Database links: Entrez Gene: 282208 Bovin Entrez Gene: 1446 Human Omim: 115450 Human SwissProt: P47710 Human Unigene: 3155 Human 酪蛋白(casein)是牛乳包括牛初乳的主要成分，通常在分离提取乳清过程中可得到大量的酪蛋白，它具有各种必需氨基酸，且组分合理，是一种优质的动物蛋白。然而，由于人体尤其是儿童对牛乳酪蛋白的分解能力较弱，牛乳酪蛋白的应用并不广泛。近来国外文献报道，牛乳酪蛋白短链肽除了易吸收外，还具有某些重要的生物学功能，提示着酪蛋白除单纯营养作用之外的广阔应用前景。 |
| 产品图片 |
Sample:
Lane 1: Recombinant Bovine Alpha s1 Casein
Primary: Anti-Alpha casein (bs-0813R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 34 kDa
25 ug total protein per lane of various lysates (see on figure) probed with Alpha casein polyclonal antibody, unconjugated (bs-0813R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
500 ng rbAlpha s1-His protein (bs-42025P) per lane probed with Alpha casein polyclonal antibody respectively, unconjugated (bs-0813R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
