| 产品编号 | bs-1288R |
| 英文名称 | GDF8 Rabbit pAb |
| 中文名称 | 生长分化因子8抗体 |
| 别 名 | GDF 8; GDF-8; GDF8_HUMAN; Growth differentiation factor 8; Growth/Differentiation Factor 8; MSTN; myostatin; OTTHUMP00000163498. |
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Specific References (5) | bs-1288R has been referenced in 5 publications.
[IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858 WB ; Mouse.
[IF=4.366] Genxi Zhang. et al. MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation. Cells-Basel. 2021 Feb;10(2):423 WB ; Chicken.
[IF=4.26] Qian et al. Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs. (2015) Sci.Re. 5:14435 WB ; Pig.
[IF=2.788] Jin, Zhengyun. et al. Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs. TRANSGENIC RES. 2022 May;:1-9 WB ; Pig.
[IF=2.59] Yamada A.K.. et al. Beta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathway. Braz J Med Biol Res. 2022 Jan;55: WB ; Rat.
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| 研究领域 | 免疫学 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12/43 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDF8: 281-375/375 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. Acts specifically as a negative regulator of skeletal muscle growth. [SUBUNIT] Homodimer; [TISSUE SPECIFICITY] Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue. Belongs to the TGF-beta family. Function: Acts specifically as a negative regulator of skeletal muscle growth. Subunit: Homodimer; disulfide-linked. Interacts with WFIKKN2, leading to inhibit its activity. Interacts with FST3. Subcellular Location: Secreted Tissue Specificity: Predominantly expressed in muscle. At hatching, expression is strongest in the skin epithelium, and is also found in the retina and brain. From day 28, expressed in skeletal muscle. In the adult, highest expression is seen in the gastrointestinal tract, brain, muscle, heart and testis. Also expressed in the adult pharynx, kidney, spleen, liver, gill, eyes, skin, swim bladder and ovary. DISEASE: Defects in MSTN are the cause of muscle hypertrophy (MSLHP) [MIM:614160]. MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Similarity: Belongs to the TGF-beta family. SWISS: O14793 Gene ID: 2660 Database links: Entrez Gene: 2660 Human Entrez Gene: 17700 Mouse Entrez Gene: 373964 Chicken Entrez Gene: 798441 Zebrafish Omim: 601788 Human SwissProt: O14793 Human SwissProt: O08689 Mouse SwissProt: O18830 Sheep SwissProt: O42222 Zebrafish Unigene: 41565 Human Unigene: 3514 Mouse Unigene: 44460 Rat GDF-8又称MSTN,是转化生长因子超家族，也是近年来发现的一类重要的肌细胞生长调控因子，它通过抑制MyoD家族成员转录活性负向控制肌细胞的生长发育，它的表达量与肌肉重量的变化呈负相关。 |
| 产品图片 |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-GDF8 (bs-1288R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/43 kD
Observed band size: 43 kD
Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Muscle (Rat) Lysate at 40 ug
Lane 3: Tongue (Rat) Lysate at 40 ug
Primary:
Anti-GDF8 (bs-1288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated(bs-1288R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
