| 产品编号 | bs-0811R |
| 英文名称 | CX3CL1 Rabbit pAb |
| 中文名称 | CX3CL1抗体 |
| 别 名 | ABCD-3; C3Xkine; CXC3; CXC3C; NTN; NTT; SCYD1; fractalkine; neurotactin; CX3C; D8Bwg0439e; FK; X3CL1_HUMAN; CX3CL1; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Small-inducible cytokine D1; FKN; X3CL1_MOUSE; X3CL1_RAT; Acc1; |
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Specific References (4) | bs-0811R has been referenced in 4 publications.
[IF=10.392] Wenlong Zhang. et al. Crosstalk between IL-15Rα+ tumor-associated macrophages and breast cancer cells reduces CD8+ T cell recruitment. CANCER COMMUN. 2022 May 25 WB ; Mouse.
[IF=5.534] Zhang J et al. Dynamic changes of CX3CL1/CX3CR1 axis during microglial activation and motor neuron loss in the spinal cord of ALS mouse model. Transl Neurodegener. 2018 Dec 21;7:35. WB ; Mouse.
[IF=5.195] Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:116640 IF ; Rat.
[IF=2.89] Xie, Wu-Ling, et al. "Abnormal Activation of Microglia Accompanied with Disrupted CX3CR1/CX3CL1 Pathway in the Brains of the Hamsters Infected with Scrapie Agent 263K." Journal of Molecular Neuroscience (2013): 1-14. WB ;
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| 研究领域 | 心血管 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41 kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CX3CL1: 301-397/397 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion. Function: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Subunit: Monomer. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Processed fractalkine: Secreted. Tissue Specificity: Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Post-translational modifications: A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. O-glycosylated with core 1 or possibly core 8 glycans. Similarity: Belongs to the intercrine delta family. SWISS: P78423 Gene ID: 6376 Database links: Entrez Gene: 6376 Human Entrez Gene: 20312 Mouse Omim: 601880 Human SwissProt: P78423 Human SwissProt: O35188 Mouse Unigene: 531668 Human 神经趋化蛋白cx3cl1免疫应答应答及细胞附着，参与细胞因子和趋化因子介导信号转导、免疫细胞趋化性、调控炎症应答反应、白细胞附着激活、正调控钙非依赖细胞附着。 |
| 产品图片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CX3CL1 Polyclonal Antibody, Unconjugated(bs-0811R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
