| 产品编号 | bs-1897R |
| 英文名称 | Synaptotagmin-4 Rabbit pAb |
| 中文名称 | 突触结合蛋白4抗体 |
| 别 名 | SYT4_HUMAN; Syt4; SytIV; HsT1192; KIAA1342; Synaptotagmin IV; SYT4; SYT 4; Syt IV; SytIV; Synaptotagmin4; SynaptotagminIV. |
| 研究领域 | 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Cow,Chicken,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Synaptotagmin-4: 301-400/425 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the synaptotagmin family. Members of this family are multi-domained, integral membrane proteins of synaptic vesicles, and are thought to serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. This gene is primarily expressed in the nervous tissues. [provided by RefSeq]. Function: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Tissue Specificity: Expressed in brain. Within brain, expression is highest in hippocampus, with substantial levels also detected in amygdala and thalamus. Similarity: Belongs to the synaptotagmin family. Contains 2 C2 domains. SWISS: Q9H2B2 Gene ID: 6860 Database links: Entrez Gene: 6860 Human Omim: 600103 Human SwissProt: Q9H2B2 Human Unigene: 8059 Human |
| 产品图片 |
Sample:
Lane1:Brain(Mouse) Lysate at 30 ug
Lane2:Heart(Mouse) Lysate at 30 ug
Primary: Anti-SYNAPTOTAGMIN-4(bs-1897R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution;
Predicted band size : 48kD
Observed band size : 47kD
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- Synaptotagmin-4 (bs-1897R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
