| 产品编号 | bs-0479R |
| 英文名称 | VSIG4 |
| 中文名称 | T淋巴细胞负调节蛋白抗体 |
| 别 名 | V-set and immunoglobulin domain-containing protein 4; CRIg; V set and immunoglobulin domain containing 4; Z39IG; VSIG4_HUMAN. |
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Specific References (1) | bs-0479R has been referenced in 1 publications.
[IF=4.123] Shi CX et al. Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways. Front Cell Infect Microbiol. 2020 May 5;10:196. WB ; Mouse.
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| 研究领域 | 免疫学 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Dog,Cow) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 33kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VSIG4: 81-160/399 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
T cell activation by APCs is positively and negatively regulated by members of the B7 family. We have identified a previously unknown function for B7 familyrelated protein V-set and Ig domaincontaining 4 (VSIG4). Administration to mice of soluble VSIG4-Ig fusion molecules reduced the induction of T cell responses in vivo and inhibited the production of Th celldependent IgG responses. Unlike that of B7 family members, surface expression of VSIG4 was restricted to resting tissue macrophages and absent upon activation by LPS or in autoimmune inflammatory foci. The specific expression of VSIG4 on resting macrophages in tissue suggests that this inhibitory ligand may be important for the maintenance of T cell unresponsiveness in healthy tissues. Function: Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. Subcellular Location: Membrane; Single-pass type I membrane protein (Probable). Tissue Specificity: Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages. Similarity: Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: Q9Y279 Gene ID: 11326 Database links: Entrez Gene: 11326 Human Entrez Gene: 278180 Mouse Omim: 300353 Human SwissProt: Q9Y279 Human Unigene: 8904 Human Unigene: 26781 Mouse Unigene: 138093 Rat |
| 产品图片 |
Sample:
Lung (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (bs-0479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD 
Sample:
Lung(Mouse)Lysate at 30 ug Placenta(Mouse) Lysate at 30 ug Primary: Anti-VSIG4 (bs-0479R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD 
Sample:
Placenta (Mouse) Lysate at 40 ug Primary: Anti-VSIG4 (bs-0479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD 
Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti- VSIG4 Polyclonal Antibody, Unconjugated(bs-0479R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
