| 产品编号 | bs-0819R |
| 英文名称 | Rabbit Anti-Tyrosinase antibody |
| 中文名称 | 酪氨酸酶抗体 |
| 别 名 | LB24 AB; LB24-AB; Monophenol monooxygenase; SK29 AB; Tumor rejection antigen AB; LB24 AB; Monophenol monooxygenase; OCA1A; OCAIA; Oculocutaneous albinism IA; SK29 AB; SK29-AB; Tumor rejection antigen AB; TYR; tyrosinase (oculocutaneous albinism IA); TYRO_HUMAN. |
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Specific References (5) | bs-0819R has been referenced in 5 publications.
[IF=4.868] Miao F et al. Intramelanocytic Acidification Plays a Role in the Antimelanogenic and Antioxidative Propertiesof Vitamin C and Its Derivatives. Oxid Med Cell Longev. 2019 May 12;2019:2084805. WB ; Human.
[IF=4.563] Kumiko Kobayashi-Nakamura. et al. Rhamnazin suppresses melanosome transport by promoting the ubiquitin-mediated proteasomal degradation of melanophilin. J Dermatol Sci. 2021 Dec;: IF ; Mouse.
[IF=4.191] Lee C. J. et al. Melanogenesis regulatory activity of the ethyl acetate fraction from Arctium lappa L. leaf on α-MSH–induced B16/F10 melanoma cells. Industrial Crops and Products,2019 138, 111581. WB ; Mouse.
[IF=4.171] Han H et al. Anti-Melanogenic Effect of Ethanolic Extract of Sorghum bicolor on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells. Nutrients. 2020 Mar 20;12(3). WB ; mouse.
[IF=4.067] Kwong SP et al. Identification of photodegraded derivatives of usnic acid with improved toxicity profile and UVA/UVB protection in normal human L02 hepatocytes and epidermal melanocytes. J Photochem Photobiol B. 2020 Feb 6;205:111814. WB ; Human.
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| 研究领域 | 肿瘤 免疫学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Tyrosinase: 155-250/529 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Tyrosinase is the key enzyme for melanin,synthesis in mammalian melanocytes and has been considered to be a unique marker for the study of melanocyte differentiation. A cDNA library was constructed from poly(A)+ mRNA from mouse melanocytes and screened using anti- tyrosinase antiserum and oligonucleotide probes corresponding to amino acid sequence of tyrosinase. sequencing of some cDNA clones positive in these screenings gave a nucleotide sequence of 1838 nucleotides including a open reading frame of 1344 nucleotides that was found to correspond exactly to the amino acid sequence of the cyanogen bromide fragments of tyrosinase. Function: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Subcellular Location: Melanosome membrane; Single-pass type I membrane protein. DISEASE: Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia. Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C. Similarity: Belongs to the tyrosinase family. SWISS: P14679 Gene ID: 7299 Database links: Entrez Gene: 7299 Human Omim: 606933 Human SwissProt: P14679 Human Unigene: 503555 Human 酪氨酸酶( Tyrosinase) 又称酚氧化酶、多酚氧化酶、儿茶酚氧化酶,是结构复杂的多亚基的含铜氧化还原酶,广泛存在于微生物、动植物及人体中。 酪氨酸酶具有独特的双重催化功能,是生物体内黑色素合成的关键酶,与人的衰老,昆虫的伤口愈合与发育,果蔬的褐变有密切关系。多年来,酪氨酸酶一直受到国内外的关注,其研究涉及生物、医学、农学、化学、药学等多个学科和领域.酪氨酸酶作为黑色素合成的关键酶,其异常过量表达可导致人体的色素沉着性疾病。 |
| 产品图片 |
Sample:
Lane 1: Eye (Mouse) Lysate at 40 ug
Lane 2: Skin (Mouse) Lysate at 40 ug
Primary: Anti-Tyrosinase (bs-0819R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 80 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-yrosinase Polyclonal Antibody, Unconjugated(bs-0819R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
