| 产品编号 | bs-0449R |
| 英文名称 | Rabbit Anti-TSLP antibody |
| 中文名称 | 胸腺基质淋巴细胞生成素-1抗体 |
| 别 名 | Thymic stromal lymphopoietin; Thymic stromal lymphopoietin isoform 1; Thymic stromal lymphopoietin protein TSLP; TSLP protein; TSLP_HUMAN. |
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Specific References (4) | bs-0449R has been referenced in 4 publications.
[IF=5.34] Dian-Dong Hou. et al. Therapeutic effects of myricetin on atopic dermatitis in vivo and in vitro. PHYTOMEDICINE. 2022 Jul;102:154200 IHC ; Mouse.
[IF=3.483] Shen Z et al. Insights into Roseburia intestinalis which alleviates experimental colitis pathology by inducing anti-inflammatory responses. J Gastroenterol Hepatol. 2018 Oct;33(10):1751-1760. IHC-P ; Mouse.
[IF=2.493] Min-jie Gong. et al. Melatonin reduces IL-33 and TSLP expression in human nasal epithelial cells by scavenging ROS directly. IMMUN INFLAMM DIS. 2023 Feb;11(2):e788 WB ; Human.
[IF=0.67] Sakitani, Eri, et al. "Increased Expression of Thymic Stromal Lymphopoietin and Its Receptor in Kimuras Disease." ORL 77.1 (2015): 44-54. other ;
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| 研究领域 | 细胞生物 免疫学 细胞因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TSP-1: 101-159/159 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Thymic stromal lymphopoietin (TSLP) is a novel cytokine that was found to promote the development of murine B cells in vitro. This protein, which is expressed in a number of tissues including heart, liver and prostate, prevented apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ-3. Anti-interleukin (IL)-7 receptor antibodies (Abs) neutralized this effect indicating that TSLP binds to at least part of the IL-7 receptor complex. TSLP induced phosphorylation of signal transducer and activator of transcription (STAT)-5. Function: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. Subunit: Interacts with a receptor composed of CRLF2 and IL7R. Subcellular Location: Secreted. Tissue Specificity: Expressed in a number of tissues including heart, liver and prostate. SWISS: Q969D9 Gene ID: 85480 Database links: Entrez Gene: 85480 Human Entrez Gene: 53603 Mouse Omim: 607003 Human SwissProt: Q969D9 Human SwissProt: Q9JIE6 Mouse Unigene: 389874 Human Unigene: 143716 Mouse Unigene: 201724 Rat 胸腺基质淋巴细胞生成素-1（胸腺基质细胞分泌的淋巴细胞生成素-1） 为一种新型促进生长的细胞因子——胸腺基质淋巴蛋白（TSLP）可以引发炎症反应， 这种炎症反应可以引起很多过敏性疾病和哮喘。 TSLP可能是过敏性疾病最重要的引发剂，已得到科学家的重视，如果证实了 TSLP是一种起始因子，抑制TSLP可以有效地治疗哮喘和其它过敏性疾病。 |
| 产品图片 |
Sample:
Lane1:K562 Cell Lysate at 30ug
Lane2: Raji Cell Lysate at 30ug
Primary: Anti-TSLP (bs-0449R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 15 kD
Observed band size: 17 kD
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TSLP Polyclonal Antibody, Unconjugated(bs-0449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSLP) Polyclonal Antibody, Unconjugated (bs-0449R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSLP) Polyclonal Antibody, Unconjugated (bs-0449R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
