| 产品编号 | bs-1202R |
| 英文名称 | TRADD |
| 中文名称 | 肿瘤坏死因子受体1相关死亡域蛋白抗体 |
| 别 名 | TNFR1 associated DEATH domain protein; TNFR1-associated DEATH domain protein; TNFRSF1A associated via death domain; TNFRSF1A-associated via death domain; tradd; TRADD_HUMAN; Tumor necrosis factor receptor type 1 associated DEATH domain protein; Tumor necrosis factor receptor type 1-associated DEATH domain protein. |
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Specific References (1) | bs-1202R has been referenced in 1 publications.
[IF=2.37] Chen, Zhiqiang, et al. "Hydrogen sulfide protects against TNF-α induced neuronal cell apoptosis through miR-485-5p/TRADD signaling." Biochemical and Biophysical Research Communications (2016). WB ; Human.
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| 研究领域 | 肿瘤 心血管 细胞生物 细胞凋亡 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of mouse TRADD: 111-230/310 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Function: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Subunit: Interacts with TRIP12. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Subcellular Location: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Note=Shuttles between the cytoplasm and the nucleus. Tissue Specificity: Found in all examined tissues. Similarity: Contains 1 death domain. SWISS: Q3U0V2 Gene ID: 8717 Database links: Entrez Gene: 8717 Human Entrez Gene: 71609 Mouse Omim: 603500 Human SwissProt: Q15628 Human SwissProt: Q3U0V2 Mouse Unigene: 460996 Human Unigene: 264255 Mouse TRADD在凋亡信号传导中起重要作用，近年来多用于研究其在肿瘤中的作用，探讨TRADD在促细胞凋亡和促细胞存活的机制如何控制，将有利于我们了解肿瘤发病机理。 |
| 产品图片 |
Sample:
Lane1: Colon carcinoma (Human)Lysate at 30 ug Lane2:Brain(Mouse) Lysate at 30 ug Primary: Anti-TRADD (bs-1202R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 35kD Observed band size : 34kD 
Sample:
Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- TRADD (bs-1202R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD 
Sample:
Jurkat(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- TRADD (bs-1202R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD 
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated(bs-1202R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated(bs-1202R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
