产品编号 | bs-0226R |
英文名称 | Rabbit Anti-Thymosin Alpha-1 antibody |
中文名称 | 胸腺肽α1抗体 |
别 名 | Prothymosin alpha (gene sequence 28); Prothymosin alpha; Prothymosin alpha like 1 protein; Thymalfasin; Thymosin alpha 1 acetate; Thymosin alpha 1; Thymosin alpha; TMSA; PTMA; PTMA_HUMAN. |
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Specific References (1) | bs-0226R has been referenced in 1 publications.
[IF=2.42] Shao, Congwen, et al. "Thymosin alpha-1-transformed< i> Bifidobacterium promotes T cell proliferation and maturation in mice by oral administration." International immunopharmacology (2013). WB ;
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研究领域 | 肿瘤 免疫学 内分泌病 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Rat (predicted: Mouse,Cow) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 12kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Thymosin Alpha-1: 2-29/111 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The thymus gland produces several hormones or hormone-like substances which are derived from a polypeptide precursor containing (in the rat) 113 amino acids and known as prothymosin alpha. A peptide containing 28 amino acid residues, named thymosin alpha 1, was originally isolated from calf thymosin fraction 5 and shown to restore various aspects of immune function in several in vitro and in vivo test systems. Thymosin alpha 1 was subsequently isolated from a similar fraction from human thymosin and reported to have the same amino acid sequence as bovine thymosin alpha 1. Function: Prothymosin alpha may mediate immune function by conferring resistance to certain opportunistic infections. Subcellular Location: Nucleus. Post-translational modifications: Covalently linked to a small RNA of about 20 nucleotides. Similarity: Belongs to the pro/parathymosin family. SWISS: P06454 Gene ID: 5757 Database links: Entrez Gene: 5757 Human Omim: 188390 Human SwissProt: P06454 Human Unigene: 459927 Human Unigene: 708083 Human 胸腺素α-1;胸腺肽 |
产品图片 |
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Thymosin Alpha-1) polyclonal Antibody, Unconjugated (bs-0026R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |