产品编号 | bs-0100R |
英文名称 | Rabbit Anti-TGF beta 2 Propeptide antibody |
中文名称 | 转移生长因子β2抗体 |
别 名 | Transforming growth factor beta-2 proprotein; TGFB2; TGF B2; BSC 1 cell growth inhibitor; BSC-1 cell growth inhibitor; BSC1 cell growth inhibitor; Cetermin; G TSF; G-TSF; Glioblastoma derived T cell suppressor; Glioblastoma derived T cell suppressor factor; Glioblastoma-derived T-cell suppressor factor; GTSF; MGC116892; Polyergin; TGF beta2; TGF-beta-2; TGF-beta2; TGFB 2; TGFB2_HUMAN; Transforming growth factor beta 2; Transforming growth factor beta-2. |
![]() |
Specific References (2) | bs-0100R has been referenced in 2 publications.
[IF=4.483] Yan Li. et al. Simvastatin inhibits POVPC-mediated induction of endothelial to mesenchymal cell transition. J Lipid Res. 2021 Mar;:100066 WB ; Human.
[IF=2.56] Xu, C., et al. "Proteomics Analysis of Hepatocyte Proliferation Regulated by FGF, PDGF, Insulin, Oncostatin M and Interleukin 2 Signaling Pathways during Rat Liver Regeneration." J Proteomics Computational Biol 1.1 (2014): 8. WB ; Rat.
|
研究领域 | 肿瘤 信号转导 生长因子和激素 转录调节因子 新陈代谢 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 12/45 kDa |
检测分子量 | |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TGF-beta 2: 154-197/414 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye. Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Subunit: Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN. Subcellular Location: Secreted. Similarity: Belongs to the TGF-beta family. SWISS: P61812 Gene ID: 7042 Database links: Entrez Gene: 7042 Human Entrez Gene: 21808 Mouse Omim: 190220 Human SwissProt: P61812 Human SwissProt: P27090 Mouse Unigene: 133379 Human Unigene: 18213 Mouse 生长因子和激素( Growth Factor and Hormones) TGF是一种多效生长因子,对上皮细胞增殖有潜在抑制作用,可抑制肿瘤生长用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。此抗体是TGFβ2 特异性抗体,与TGF-β1和TGF-β3没有交叉反应。 |
产品图片 |
Sample:Kidney(Mouse)Lysate at 40 ug
Primary: Anti-TGF beta 2 Propeptide(bs-0100R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12'45kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti- TGF beta 2 Propeptide (bs-0100R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/45 kDa
Observed band size: 48 kDa
Sample:MCF-7 (Human)Cell Lysate at 40 ug
Primary: Anti-TGF beta 2 Propeptide(bs-0100R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12'45kD
Observed band size: 48kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta-2 Polyclonal Antibody, Unconjugated(bs-0100R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |