| 产品编号 | bs-0121R |
| 英文名称 | SYAP1 Rabbit pAb |
| 中文名称 | 突触相关蛋白-1抗体 |
| 别 名 | Synapse associated protein-1; synapse-associated protein 1-like; 2010110O17Rik, AW011796, DKFZp686K221, FLJ14495, FLJ44185, MGC94115, PRO3113, SYAP1; SYAP1_HUMAN; SAP-1. |
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Specific References (2) | bs-0121R has been referenced in 2 publications.
[IF=15.84] Han Yang. et al. A Novel Targeted and High‐Efficiency Nanosystem for Combinational Therapy for Alzheimer's Disease. Adv Sci. 2020 Oct;7(19):1902906 IF,FC ; Mouse.
[IF=2.88] Gao, Yuhua, et al. "Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells." BioMed Research International 2013 (2013). Chicken.
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| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SAP-1 : 151-230/352 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SYAP1 (synapse-associated protein 1) is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is a novel domain that is present in basal transcription factors, synapse-associated proteins and several hypothetical proteins. The BSD domain is characterized by three predicted alpha helices and by conserved tryptophan and phenylalanine residues, located at the C-terminus of the domain. The gene that encodes SYAP1 in humans is located on chromosome X. Chromosome X consists of about 153 million base pairs and nearly 1,000 genes. Color blindness, hemophilia, and Duchenne muscular dystrophy are well known X chromosome-linked conditions which affect males more frequently as males carry a single X chromosome. Similarity: Contains 1 BSD domain. SWISS: Q96A49 Gene ID: 94056 Database links: Entrez Gene: 94056 Human Entrez Gene: 67043 Mouse SwissProt: Q96A49 Human SwissProt: Q9D5V6 Mouse Unigene: 729197 Human Unigene: 44207 Mouse 突触素SAP-1/SYP(Synaptophysin, FRG-4)是分布于突触囊泡膜上含量丰富的糖蛋白之一，与递质释放关系密切，并可影响突触可塑性。突触相关蛋白-1可作为神经元和神经内分泌细胞的可靠标记物用于神经系统形态、功能和发育以及相关疾病的研究。对于SAP-1的功能及其具体作用机制，尚待进一步研究。 |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SYP p38/Synaptophysin/SAP-1 Polyclonal Antibody, Unconjugated(bs-0121R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hep G2 (blue).
Primary Antibody (green line): Rabbit Anti-SYAP1 antibody (bs-0121R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
